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Ubiquitin Conjugation Factor E4 B Antibody (YA3308)

(Synonyms: E4; UFD2; HDNB1; UBOX3; UFD2A)
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Based on 1 Customer Validation

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugation Factor E4 B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ubiquitin Conjugation Factor E4 B Antibody (YA3308)
  • Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugation Factor E4 B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 146 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 146 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human UBE4B aa28-40.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ubiquitin Conjugation Factor E4 B Antibody (YA3308)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HL-60 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), HepG2 (lane 4, 15 μg), C2C12 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using Ubiquitin Conjugation Factor E4 B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Ubiquitin Conjugation Factor E4 B is an Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation[1].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Expressed in differentiated myotubes (at protein level) (PubMed:17369820) . Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788) . Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788) . Almost undetectable in lung and spleen (PubMed:11802788)

  • Isoforms & Post-Translational Modification

    O95155 has 4 isomers: O95155-1: 146185 Da (predicted); O95155-2: 133275 Da (predicted); O95155-3: 121820 Da (predicted); O95155-4: 151561 Da (predicted).
    Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7

  • Subunit

    Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B

  • SwissProt ID

    O95155

  • Gene ID
    10277 [NCBI]
  • Synonyms

    E4; UFD2; HDNB1; UBOX3; UFD2A

  • Research Field

    -

References

[1]. Janiesch PC, et al. The ubiquitin-selective chaperone CDC-48/p97 links myosin assembly to human myopathy. Nat Cell Biol. 2007 Apr;9(4):379-90. [Content Brief]

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HL-60 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), HepG2 (lane 4, 15 μg), C2C12 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using Ubiquitin Conjugation Factor E4 B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Product Image
    1/16
  • Experimental Validation Results for Ubiquitin Conjugation Factor E4 B Antibody (YA3308)
  • Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Product Image
  • Experimental Validation Results for Ubiquitin Conjugation Factor E4 B Antibody (YA3308)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HL-60 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), HepG2 (lane 4, 15 μg), C2C12 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using Ubiquitin Conjugation Factor E4 B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ubiquitin Conjugation Factor E4 B Antibody (YA3308)
  • Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HL-60 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), HepG2 (lane 4, 15 μg), C2C12 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using Ubiquitin Conjugation Factor E4 B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Product Image WB