Ubiquitin Conjugation Factor E4 B Antibody (YA3308)

Cat. No.: HY-P83563: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugation Factor E4 B.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugation Factor E4 B.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 146 kDa;

Observed band size: 146 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

O95155

Gene ID

10277 [NCBI]

Immunogen

A synthetic peptide of human UBE4B aa28-40.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HL-60 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), HepG2 (lane 4, 15 μg), C2C12 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using Ubiquitin Conjugation Factor E4 B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Expressed in differentiated myotubes (at protein level) (PubMed:17369820) . Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788) . Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788) . Almost undetectable in lung and spleen (PubMed:11802788)

Isoforms & Post-Translational Modification：O95155 has 4 isomers: O95155-1: 146185 Da (predicted); O95155-2: 133275 Da (predicted); O95155-3: 121820 Da (predicted); O95155-4: 151561 Da (predicted).
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7

Subunit：Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B

Database

Entrez Gene: 10277 Human ; 63958 Mouse ; 298652 Rat

SwissProt: O95155 Human ; Q9ES00 Mouse ;

OMIM: 613565 Human

Research Field

Synonyms

E4; UFD2; HDNB1; UBOX3; UFD2A

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Ubiquitin Conjugation Factor E4 B Antibody (YA3308)

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Featured Recommendations:

Product Detail

Verification Image

Background

Documentation

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report