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SOD2 Antibody (YA670)

(Synonyms: IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD; SOD2)
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Based on 1 Customer Validation

SOD2 Antibody (YA670) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SOD2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SOD2 Antibody (YA670)
  • SOD2 Antibody (YA670) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

SOD2 Antibody (YA670) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SOD2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database

Entrez Gene: 6648 Human

SwissProt: P04179 Human

Immunogen

Synthetic peptide corresponding to Human SOD2.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102742

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SOD2 Antibody (YA670)
    Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using SOD2 (HY-P80898) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SOD2 is a Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Induction:Expression is regulated by KRIT1

  • Isoforms & Post-Translational Modification

    P04179 has 4 isomers: P04179-1: 24750 Da (predicted); P04179-2: 20724 Da (predicted); P04179-3: 18262 Da (predicted); P04179-4: 19730 Da (predicted).
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity;Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity);Polyubiquitinated; leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability

  • Subunit

    Homotetramer

  • SwissProt ID

    P04179

  • Gene ID
    6648 [NCBI]
  • Synonyms

    IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD; SOD2

  • Research Field

    Cell Biology

SOD2 Antibody (YA670) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using SOD2 (HY-P80898) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SOD2 Antibody (YA670) Product Image
    1/16
  • Experimental Validation Results for SOD2 Antibody (YA670)
  • SOD2 Antibody (YA670) Product Image
  • Experimental Validation Results for SOD2 Antibody (YA670)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using SOD2 (HY-P80898) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SOD2 Antibody (YA670)
  • SOD2 Antibody (YA670) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg) and K562 (lane 3(40μg) using SOD2 (HY-P80898) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SOD2 Antibody (YA670) Product Image WB