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Cat. No.: HY-P80531A

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Acetyl-Histone H3 (Lys4) Antibody (YA641)(PBS only)

Name: Acetyl-Histone H3 (Lys4) Antibody (YA641)(PBS only)
Brand: MedChemExpress
SKU: HY-P80531A
Availability: PreOrder

(Synonyms: H3K4ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J)

Acetyl-Histone H3 (Lys4) Antibody (YA641) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys4).

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence
Dilution Ratio	1:500-1:1000	1:50-1:200

Description

Acetyl-Histone H3 (Lys4) Antibody (YA641) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys4).

Host Rabbit
Species Reactivity
Human, Mouse
Observed Molecular Weight

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 15 kDa

Immunogen

Synthetic peptide corresponding to residues surrounding Lys4 of Human Human Histone H3.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Appearance

Solution
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Function

Acetyl-Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
Subcellular Localization

Nucleus; Chromosome
Subunit

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)
SwissProt ID

P68431
Gene ID

8350 [NCBI]
Synonyms

H3K4ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
Research Field

Epigenetics and Nuclear Signaling