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Acetyl-Histone H3 (Lys4) Antibody (YA641)

(Synonyms: H3K4ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J)
Customer Review

Based on 1 Customer Validation

Acetyl-Histone H3 (Lys4) Antibody (YA641) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys4).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Acetyl-Histone H3 (Lys4) Antibody (YA641) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys4).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to residues surrounding Lys4 of Human Human Histone H3.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Acetylated

Isotype

IgG

RRID

AB_3102120

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
    Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2, Negative), HeLa (lane 3, Trichostatin A (HY-15144), 0.1 μM, 4 h), HeLa (lane 4, Trichostatin A (HY-15144), 0.5 μM, 4 h), and HeLa (lane 5, Trichostatin A (HY-15144), 1 μM, 4 h) using Acetyl-Histone H3 (Lys4) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Acetyl-Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)

  • SwissProt ID

    P68431

  • Gene ID
    8350 [NCBI]
  • Synonyms

    H3K4ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

  • Research Field

    Epigenetics and Nuclear Signaling

Acetyl-Histone H3 (Lys4) Antibody (YA641) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2, Negative), HeLa (lane 3, Trichostatin A (HY-15144), 0.1 μM, 4 h), HeLa (lane 4, Trichostatin A (HY-15144), 0.5 μM, 4 h), and HeLa (lane 5, Trichostatin A (HY-15144), 1 μM, 4 h) using Acetyl-Histone H3 (Lys4) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image
    1/16
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2, Negative), HeLa (lane 3, Trichostatin A (HY-15144), 0.1 μM, 4 h), HeLa (lane 4, Trichostatin A (HY-15144), 0.5 μM, 4 h), and HeLa (lane 5, Trichostatin A (HY-15144), 1 μM, 4 h) using Acetyl-Histone H3 (Lys4) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys4) Antibody (YA641)
  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2, Negative), HeLa (lane 3, Trichostatin A (HY-15144), 0.1 μM, 4 h), HeLa (lane 4, Trichostatin A (HY-15144), 0.5 μM, 4 h), and HeLa (lane 5, Trichostatin A (HY-15144), 1 μM, 4 h) using Acetyl-Histone H3 (Lys4) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image WB
  • Acetyl-Histone H3 (Lys4) Antibody (YA641) Product Image WB