1. Cell Cycle/DNA Damage
    Epigenetics
  2. HDAC

Trichostatin A (Synonyms: TSA)

Cat. No.: HY-15144 Purity: 99.53%
Handling Instructions

Trichostatin A is a potent and specific inhibitor of histone deacetylase (HDAC) activity with IC50 value of 1.8 nM.

For research use only. We do not sell to patients.
Trichostatin A Chemical Structure

Trichostatin A Chemical Structure

CAS No. : 58880-19-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 198 In-stock
2 mg USD 119 In-stock
5 mg USD 226 In-stock
10 mg USD 408 In-stock
25 mg USD 840 In-stock
50 mg USD 1440 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

    Trichostatin A purchased from MCE. Usage Cited in: Proc Natl Acad Sci U S A. 2016 Sep 6;113(36):9967-76.

    SDL screens for TDP1 and validation pipeline. Acetylation levels after treatment with TSA in RMS (RH30) cells.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Trichostatin A is a potent and specific inhibitor of histone deacetylase (HDAC) activity with IC50 value of 1.8 nM.

    IC50 & Target

    IC50: 1.8 nM (HDAC)[1]

    In Vitro

    Trichostatin A (TSA) inhibits proliferation of eight breast carcinoma cell lines with mean±SD IC50 of 124.4±120.4 nM (range, 26.4-308.1 nM). HDAC inhibitory activity of TSA is similar in all cell lines with mean±SD IC50 of 2.4±0.5 nM (range, 1.5-2.9 nM)[1]. Trichostatin A (330 nM) increases Gαs protein expression in human myometrial cells, but does not increase Gαs mRNA levels[2]. Trichostatin A (20-75 nM) induces minimal cytotoxicity to adipose-derived stem cells (ADSCs), and enhances the osteogenic differentiation capacity of ADSCs[3].

    In Vivo

    Trichostatin A (500 μg/kg, s.c.) pronounces antitumor activity without causing any measurable toxicity in doses of up to 5 mg/kg by s.c. injection, in randomized controlled efficacy studies using the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model[1].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 3.3072 mL 16.5360 mL 33.0721 mL
    5 mM 0.6614 mL 3.3072 mL 6.6144 mL
    10 mM 0.3307 mL 1.6536 mL 3.3072 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [1]

    Briefly, 20 μL of crude cell extract (-2.5 × 105 cells), in the presence of varying concentrations of Trichostatin A (TSA) in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 min at 25°C with 1 μL (-1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    Trichostatin A is dissolved in absolute ethanol at a concentration of 10 mM. 

    Stock cultures of breast cancer cell lines MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 are grown in DMEM containing 10% (v/v) FCS, 2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin at 37°C in 5% CO2 humidified atmosphere. Cells are counted in a hemocytometer after detachment using 0.25% (w/v) trypsin in Dulbecco’s PBS without Ca2+ or Mg2+ containing 0.02% (w/v) EDTA. Viability is determined by trypan blue exclusion. For each cell line, cells are seeded in 96-well microtiter plates at optimal densities determined in prior experiments to ensure exponential growth for the duration of the assay. After a 24-h preincubation, growth medium is replaced with experimental medium containing Trichostatin A (TSA) at final concentrations ranging from 10−12 M to 10−5 M in log dilutions and 0.1% (v/v) ethanol, or growth medium containing 0.1% (v/v) ethanol as a vehicle control. After 96 h incubation, cell proliferation is estimated using the sulforhodamine B colorimetric assay, and the results are expressed as the mean±SD for six replicates as a percentage of vehicle control (taken as 100%). MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Trichostatin A is dissolved in DMSO at a concentration of 2 mg/mL.

    Twelve rats are randomized to receive 500 μg/kg Trichostatin A (TSA) in 50 μL DMSO, or 50 μL DMSO as vehicle control, by s.c. injection twice weekly for 4 weeks. In subsequent studies, 30 rats are randomized to receive TSA 500 μg/kg in 50 μL DMSO, or 50 μL DMSO as vehicle control, by s.c. injection daily for 4 weeks. Weekly tumor measurements, estimated tumor volumes, and body mass are recorded for each animal. Animals are sacrificed at the end of the 4-week study period; palpable tumors are resected and immediately snap-frozen in liquid nitrogen. Animals with tumors <2 cm in diameter or ulcerating tumors are withdrawn from study. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    302.37

    Formula

    C₁₇H₂₂N₂O₃

    CAS No.

    58880-19-6

    SMILES

    CN(C1=CC=C(C=C1)C([[email protected]@H](/C=C(/C=C/C(NO)=O)C)C)=O)C

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 30 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.53%

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    Product Name:
    Trichostatin A
    Cat. No.:
    HY-15144
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