1. Metabolic Enzyme/Protease
    Epigenetics
    Cell Cycle/DNA Damage
  2. Endogenous Metabolite
    Sirtuin
  3. Nicotinamide

Nicotinamide (Synonyms: Niacinamide; Nicotinic acid amide)

Cat. No.: HY-B0150 Purity: 99.86%
Handling Instructions

Nicotinamide is a form of vitamin B3 that plays essential roles in cell physiology through facilitating NAD+ redox homeostasis and providing NAD+ as a substrate to a class of enzymes that catalyze non-redox reactions. Nicotinamide is an inhibitor of SIRT1.

For research use only. We do not sell to patients.

Nicotinamide Chemical Structure

Nicotinamide Chemical Structure

CAS No. : 98-92-0

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
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5 g USD 78 In-stock
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Based on 13 publication(s) in Google Scholar

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Description

Nicotinamide is a form of vitamin B3 that plays essential roles in cell physiology through facilitating NAD+ redox homeostasis and providing NAD+ as a substrate to a class of enzymes that catalyze non-redox reactions. Nicotinamide is an inhibitor of SIRT1.

IC50 & Target[4]

PARP-1

 

Human Endogenous Metabolite

 

In Vitro

Pretreatment with the poly (ADP-ribose) polymerase (PARP) inhibitor nicotinamide is able to prevent HCN2 cell death. When nicotinamide is added prior to t-BuOOH, it is able to prevent neuronal cell death and inhibit apoptosis. Nicotinamide-pretreated neurons have higher expression levels of inhibitors of apoptosis (IAP) genes[1]. Nicotinamide inhibits vasoconstriction by ET. Nicotinamide also alleviates oxidative stress, which exacerbates PE and FGR[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Normal and streptozotocin-nicotinamide induced adult male diabetic rats receive quercetin (10, 25 and 50 mg/kg/bw) orally, and cause significant decrease in FBG and cardiac injury marker levels with increased in insulin levels[2]. Nicotinamide improves maternal hypertension, proteinuria, and glomerular endotheliosis in RUPP mice. Moreover, nicotinamide prolongs pregnancies, and improves survival and growth of the embryos in RUPP PE mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

122.12

Formula

C₆H₆N₂O

CAS No.
SMILES

O=C(C1=CC=CN=C1)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (818.87 mM)

H2O : ≥ 50 mg/mL (409.43 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.1887 mL 40.9433 mL 81.8867 mL
5 mM 1.6377 mL 8.1887 mL 16.3773 mL
10 mM 0.8189 mL 4.0943 mL 8.1887 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (818.87 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Animal Administration
[2]

DM is induced via a single intraperitoneal (i.p) injection of nicotinamide (110 mg/kg/body weight) dissolved in normal saline 15 min prior to streptozotocin (STZ) (55 mg/kg/body weight) injection, which is dissolved in a freshly prepared 0.1mol/Lcitrate buffer (pH 4.5). These injections are given following an overnight fast. Control rats (n=6) are injected with the same amount of solvent. In order to prevent hypoglycemia in the first 24 h following STZ injection, rats are allowed to have free access to water with 5% dextrose (D5W). Three days after STZ-nicotinamide injection, rats with FBG levels greater than 7.0 mM are considered as diabetic.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.86%

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Keywords:

NicotinamideNiacinamide Nicotinic acid amideEndogenous MetaboliteSirtuinInhibitorinhibitorinhibit

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Nicotinamide
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