1. Stem Cell/Wnt Metabolic Enzyme/Protease Epigenetics Cell Cycle/DNA Damage Anti-infection
  2. Organoid Endogenous Metabolite Sirtuin HBV
  3. Nicotinamide

Nicotinamide  (Synonyms: Niacinamide; Nicotinic acid amide)

Cat. No.: HY-B0150 Purity: 99.87%
COA Handling Instructions

Nicotinamide is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide also inhibits SIRT1. Nicotinamide increases cellular NAD+, ATP, ROS levels. Nicotinamide inhibits tumor growth and improves survival. Nicotinamide also has anti-HBV activity.

For research use only. We do not sell to patients.

Nicotinamide Chemical Structure

Nicotinamide Chemical Structure

CAS No. : 98-92-0

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10 mM * 1 mL in DMSO
ready for reconstitution
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1 g USD 35 In-stock
5 g USD 50 In-stock
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Based on 35 publication(s) in Google Scholar

Other Forms of Nicotinamide:

Top Publications Citing Use of Products

35 Publications Citing Use of MCE Nicotinamide

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Description

Nicotinamide is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide also inhibits SIRT1. Nicotinamide increases cellular NAD+, ATP, ROS levels. Nicotinamide inhibits tumor growth and improves survival. Nicotinamide also has anti-HBV activity[1][2][3][4].

IC50 & Target[1][2]

Human Endogenous Metabolite

 

SIRT2

2 μM (EC50)

SIRT1

50-180 μM (IC50)

In Vitro

Nicotinamide (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells[1].
Nicotinamide (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase[1].
Nicotinamide (1-50 mM, 6 h ) increases NAD+, ATP and ROS levels in A375 and SK-MEL-28 cells[1].
Nicotinamide Hydrochloride (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC50 of 2 μM[1].
Nicotinamide (0-64 mM) inhibits HBV replication in HepAD38 and HepG2.2.15 cells[3].
Nicotinamide (10 mM, on day 13) promotes pancreatic cell differentiation from human embryonic stem cells (hESCs) through CK1 and ROCK inhibition[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A375, SK-MEL-28, mouse B16-F10 cell
Concentration: 0, 1, 20, 50 mM
Incubation Time: 24 h, 48 h
Result: Reduced cell number in a dose-dependent manner with a strong inhibitory effect at 20mM and an almost complete effect at 50 mM.

Cell Cycle Analysis[1]

Cell Line: A375, SK-MEL-28
Concentration: 10, 20, 50 mM
Incubation Time: 24 h
Result: Arrested A375 cells in G1 phase.
In Vivo

Nicotinamide Hydrochloride (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model[1].
Nicotinamide Hydrochloride (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of Eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10[1].
Nicotinamide Hydrochloride (vein injection, 0-200 mg/kg, 5 days) inhibits HBV replication in HBV-transgenic mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (subcutaneous injected with B16-F10 cells)[1]
Dosage: 1000, 1500, 1800 mg/Kg.
Administration: Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result: Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight.
Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.
Animal Model: HBV-transgenic mice[3]
Dosage: 0-200 mg/kg
Administration: Vein injection, 5 days
Result: Reduced serum HBV DNA.
Clinical Trial
Molecular Weight

122.12

Formula

C6H6N2O

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CN=C1)N

Structure Classification
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 1 years; -20°C, 6 months (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (409.43 mM; Need ultrasonic)

H2O : ≥ 50 mg/mL (409.43 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.1887 mL 40.9433 mL 81.8867 mL
5 mM 1.6377 mL 8.1887 mL 16.3773 mL
10 mM 0.8189 mL 4.0943 mL 8.1887 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (818.87 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.87%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nicotinamide
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HY-B0150
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