1. Nicotinamide Hydrochloride

Nicotinamide Hydrochloride  (Synonyms: Niacinamide Hydrochloride; Nicotinic acid amide Hydrochloride)

Cat. No.: HY-B0150A
Handling Instructions

Nicotinamide Hydrochloride is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide Hydrochloride also inhibits SIRT1. Nicotinamide Hydrochloride increases cellular NAD+, ATP, ROS levels. Nicotinamide Hydrochloride inhibits tumor growth and improves survival. Nicotinamide Hydrochloride also has anti-HBV activity.

For research use only. We do not sell to patients.

Nicotinamide Hydrochloride Chemical Structure

Nicotinamide Hydrochloride Chemical Structure

CAS No. : 25334-23-0

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Other In-stock Forms of Nicotinamide Hydrochloride:

Other Forms of Nicotinamide Hydrochloride:

Top Publications Citing Use of Products

36 Publications Citing Use of MCE Nicotinamide Hydrochloride

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Description

Nicotinamide Hydrochloride is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide Hydrochloride also inhibits SIRT1. Nicotinamide Hydrochloride increases cellular NAD+, ATP, ROS levels. Nicotinamide Hydrochloride inhibits tumor growth and improves survival. Nicotinamide Hydrochloride also has anti-HBV activity[1][2][3][4].

In Vitro

Nicotinamide Hydrochloride (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells[1].
Nicotinamide Hydrochloride (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase[1].
Nicotinamide Hydrochloride (1-50 mM, 6 h ) increases NAD+, ATP and ROS levels in A375 and SK-MEL-28 cells[1].
Nicotinamide Hydrochloride (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC50 of 2 μM[1].
Nicotinamide Hydrochloride (0-64 mM) inhibits HBV replication in HepAD38 and HepG2.2.15 cells[3].
Nicotinamide Hydrochloride (10 mM, on day 13) promotes pancreatic cell differentiation from human embryonic stem cells (hESCs) through CK1 and ROCK inhibition[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A375, SK-MEL-28, mouse B16-F10 cell
Concentration: 0, 1, 20, 50 mM
Incubation Time: 24 h, 48 h
Result: Reduced cell number in a dose-dependent manner with a strong inhibitory effect at 20mM and an almost complete effect at 50 mM.

Cell Cycle Analysis[1]

Cell Line: A375, SK-MEL-28
Concentration: 10, 20, 50 mM
Incubation Time: 24 h
Result: Arrested A375 cells in G1 phase.
In Vivo

Nicotinamide Hydrochloride (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model[1].
Nicotinamide Hydrochloride (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of Eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10[1].
Nicotinamide Hydrochloride (vein injection, 0-200 mg/kg, 5 days) inhibits HBV replication in HBV-transgenic mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (subcutaneous injected with B16-F10 cells)[1]
Dosage: 1000, 1500, 1800 mg/Kg.
Administration: Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result: Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight.
Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.
Animal Model: HBV-transgenic mice[3]
Dosage: 0-200 mg/kg
Administration: Vein injection, 5 days
Result: Reduced serum HBV DNA.
Clinical Trial
Molecular Weight

158.59

Formula

C6H7ClN2O

CAS No.
SMILES

O=C(C1=CC=CN=C1)N.[H]Cl

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nicotinamide Hydrochloride
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