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TIPRL Antibody (YA7848)

(Synonyms: TIP; TIP41)
Customer Review

Based on 1 Customer Validation

TIPRL Antibody (YA7848) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIPRL.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TIPRL Antibody (YA7848)
  • TIPRL Antibody (YA7848) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:100

Product Details

Description

TIPRL Antibody (YA7848) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIPRL.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 31.4 kDa
Immunogen

Full length human recombinant protein of human TIPRL produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TIPRL Antibody (YA7848)
    Western blot analysis of extracts from K562(lane 2, 20μg), HEK293T(lane 3, 20μg), NIH-3T3(lane 4, 20μg) and HeLa(lane 5, 20μg) using TIPRL Antibody (HY-P88164). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

Background

  • Function

    TIPRL may be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. Acts as a negative regulator of serine/threonine-protein phosphatase 4 probably by inhibiting the formation of the active PPP4C:PPP4R2 complex; the function is proposed to implicate it in DNA damage response by promoting H2AX phosphorylated on Ser-140 (gamma-H2AX). May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    O75663 has two isomers: O75663-1: 31444 Da (predicted); O75663-2: 20214 Da (predicted).

  • Subunit

    Isoform 1 interacts with PPP2CA

  • SwissProt ID

    O75663

  • Synonyms

    TIP; TIP41

TIPRL Antibody (YA7848) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2, 20μg), HEK293T(lane 3, 20μg), NIH-3T3(lane 4, 20μg) and HeLa(lane 5, 20μg) using TIPRL Antibody (HY-P88164). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • TIPRL Antibody (YA7848) Product Image
    1/16
  • Experimental Validation Results for TIPRL Antibody (YA7848)
  • TIPRL Antibody (YA7848) Product Image
  • Experimental Validation Results for TIPRL Antibody (YA7848)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2, 20μg), HEK293T(lane 3, 20μg), NIH-3T3(lane 4, 20μg) and HeLa(lane 5, 20μg) using TIPRL Antibody (HY-P88164). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIPRL Antibody (YA7848)
  • TIPRL Antibody (YA7848) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2, 20μg), HEK293T(lane 3, 20μg), NIH-3T3(lane 4, 20μg) and HeLa(lane 5, 20μg) using TIPRL Antibody (HY-P88164). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • TIPRL Antibody (YA7848) Product Image WB