G15 Antibody
(Synonyms: H37, LUCA15, RBM5, RNA-binding protein 5, Protein G15, Putative tumor suppressor LUCA15, RNA-binding motif protein 5, Renal carcinoma antigen NY-REN-9)G15 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to G15.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
G15 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to G15.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 125 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 92 kDa
Synthetic peptide corresponding to the center region of human G15.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
G15 is a Component of the spliceosome A complex. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate apoptosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis[1].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines. -
Isoforms & Post-Translational Modification
G15 has 5 isoforms, P52756-1: amino acid length is 815, molecular weight is 92154 Da (predicted); P52756-2: amino acid length is 143, molecular weight is 17055 Da (predicted); P52756-3: amino acid length is 126, molecular weight is 14897 Da (predicted); P52756-4: amino acid length is 141, molecular weight is 16838 Da (predicted); P52756-5: amino acid length is 150, molecular weight is 17891 Da (predicted).
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Subunit
Component of the spliceosome A complex (also known as the prespliceosome).
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SwissProt ID
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Synonyms
H37, LUCA15, RBM5, RNA-binding protein 5, Protein G15, Putative tumor suppressor LUCA15, RNA-binding motif protein 5, Renal carcinoma antigen NY-REN-9
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