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IL-3RB Antibody(YA3396)

(Synonyms: CSF2RB; IL3RB; IL5RB; SMDP5; CDw131; CD131; betaGMR)
Customer Review

Based on 1 Customer Validation

IL-3RB Antibody(YA3396) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-3RB.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • IL-3RB Antibody(YA3396) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:100-1:200 1:50-1:100 1:10000

Product Details

Description

IL-3RB Antibody(YA3396) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IL-3RB.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97 kDa
Species Reactivity Database
Immunogen

Purified recombinant fragment of human CD131 expressed in E. Coli.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3676602

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    Flow cytometric analysis of 1X106 Jurkat cells labeling IL-3RB Antibody (HY-P83661, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    IL-3RB cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodimeric receptor through interaction with different partners such as IL3RA, IL5RA or CSF2RA. In turn, participates in various signaling pathways including interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, interacts constitutively with JAK1 and ligand binding leads to JAK1 stimulation and subsequent activation of the JAK-STAT pathway[1][2].

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Isoforms & Post-Translational Modification

    P32927 has 2 isomers: P32927-1: 97336 Da (predicted); P32927-2: 97976 Da (predicted).
    May be phosphorylated by LYN

  • Subunit

    Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors (PubMed:1495999). The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. Interacts with TMEM102; this interaction occurs preferentially in the absence of CSF2. Interacts with FCER1G; this interaction is direct. Interacts with LYN. Interacts with JAK1 (PubMed:9516124)

  • SwissProt ID

    P32927

  • Gene ID
    1439 [NCBI]
  • Synonyms

    CSF2RB; IL3RB; IL5RB; SMDP5; CDw131; CD131; betaGMR

IL-3RB Antibody(YA3396) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling IL-3RB Antibody (HY-P83661, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • IL-3RB Antibody(YA3396) Product Image
    1/16
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • IL-3RB Antibody(YA3396) Product Image
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling IL-3RB Antibody (HY-P83661, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • Experimental Validation Results for IL-3RB Antibody(YA3396)
  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using IL-3RB Antibody(YA3396). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P83661, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IL-3RB Antibody(YA3396) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling IL-3RB Antibody (HY-P83661, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • IL-3RB Antibody(YA3396) Product Image IHC-P
  • IL-3RB Antibody(YA3396) Product Image IHC-P
  • IL-3RB Antibody(YA3396) Product Image IHC-P
  • IL-3RB Antibody(YA3396) Product Image IHC-P
  • IL-3RB Antibody(YA3396) Product Image IHC-P
  • IL-3RB Antibody(YA3396) Product Image IHC-P
  • IL-3RB Antibody(YA3396) Product Image FC