Tyrosine Hydroxylase Antibody (YA5393)

Art. -Nr.: HY-P85701: Data Sheet COA
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User Guide for Antibodies
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Tyrosine Hydroxylase Antibody (YA5393) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Tyrosine Hydroxylase.

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1 Publications Citing Use of MCE Tyrosine Hydroxylase Antibody (YA5393)

•Mol Psychiatry. 2026 Mar 23.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

Tyrosine Hydroxylase Antibody (YA5393) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Tyrosine Hydroxylase.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 58 kDa;

Observed band size: 59 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P07101

Gene ID

7054 [NCBI]

Immunogen

Synthesized peptide derived from human Tyrosine Hydrolase AA range: 1-100

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Reinheit	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1,IgG/Kappa

Appearance

Solution

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293(lane 2(20μg), SH-SY5Y(lane 3(20μg), 3T3(lane 4(20μg), PC-3(lane 5(20μg) using Tyrosine Hydroxylase Antibody (HY-P85701). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:15287903, PubMed:1680128, PubMed:17391063, PubMed:24753243, PubMed:34922205, PubMed:8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity); Lacks catalytic activity; Lacks catalytic activity

Subcellular Localization：Cytoplasm, perinuclear region; Nucleus; Cell projection, axon; Cytoplasm; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle

Expression：
Tissue_specificity:Primarily expressed in the brain and adrenal glands

Isoforms & Post-Translational Modification：P07101 has 6 isomers: P07101-1: 58600 Da (predicted); P07101-2: 58160 Da (predicted); P07101-3: 55612 Da (predicted); P07101-4: 56052 Da (predicted); P07101-5: 45338 Da (predicted); P07101-6: 44898 Da (predicted).
Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity (PubMed:7901013). Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines (PubMed:15287903). Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway (By similarity). Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network (PubMed:28637871). Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG; this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669). Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity (By similarity)

Subunit：Homotetramer (PubMed:24947669, Ref.18). Interacts (when phosphorylated at Ser-19) with YWHAG; one YWHAG dimer binds to one TH tetramer and this interaction may influence the phosphorylation and dephosphorylation of other sites (PubMed:24947669).

RRID

AB_3719034

Synonyms

Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH;

Dokumentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)