MARK3 Antibody (YA2150)

Cat. No.: HY-P82405: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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MARK3 Antibody (YA2150) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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MARK3 Antibody (YA2150) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

MARK3 Antibody (YA2150) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MARK3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 84 kDa;

Observed band size: 84 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Human

SwissProt ID

P27448

Gene ID

4140 [NCBI]

Immunogen

A synthesized peptide derived from human MARK3 aa550-600/753.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:30
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from C2C12 (lane 2(20μg), HEK293 (lane 3(20μg), MCF-7 (lane 4(20μg), SH-SY5Y (lane 5(20μg) using MARK3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Serine/threonine-protein kinase (PubMed:16822840, PubMed:16980613, PubMed:23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Phosphorylates CDC25C on 'Ser-216' (PubMed:12941695). Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol (PubMed:16822840). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714). Phosphorylates PKP2 and KSR1 (PubMed:12941695)

Subcellular Localization：Cell membrane; Peripheral membrane protein; Cell projection, dendrite; Cytoplasm

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：P27448 has 7 isomers: P27448-5: 84429 Da (predicted); P27448-2: 83955 Da (predicted); P27448-3: 81439 Da (predicted); P27448-4: 83248 Da (predicted); P27448-6: 79876 Da (predicted); P27448-7: 85381 Da (predicted); P27448-8: 74091 Da (predicted).
Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity (PubMed:15084291)

Subunit：Interacts with MAPT/TAU (PubMed:23666762). Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5 (PubMed:28087714). Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ (PubMed:16959763). Interacts with PKP2 (via N-terminus) (PubMed:12941695). Interacts with CDC25C (PubMed:12941695). Interacts with KSR1 (PubMed:12941695)

Database

Entrez Gene: 4140 Human ; 17169 Mouse ;

SwissProt: P27448 Human ; Q03141 Mouse ;

OMIM: 618283 Human

Research Field

Signal Transduction

Synonyms

C-TAK1 antibody; Cdc25C associated protein kinase 1 antibody; Cdc25C-associated protein kinase 1 antibody; cTAK1 antibody; ELKL motif kinase 2 antibody; EMK-2 antibody; Emk2 antibody; ETK 1 antibody; KIAA4230 antibody; KP78 antibody; MAP microtubule affinity regulating kinase 3 antibody; MAP/microtubule affinity-regulating kinase 3 antibody; Mark3 antibody; MARK3_HUMAN antibody; Par 1a antibody; PAR1A antibody; Protein kinase STK10 antibody; Ser/Thr protein kinase PAR-1 antibody; Serine threonine protein kinase p78 antibody; Serine/threonine-protein kinase p78 antibody; SerThr protein kinase PAR 1 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

MARK3 Antibody (YA2150) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MARK3 Antibody (YA2150)

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MARK3 Antibody (YA2150) Related Classifications

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