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DMT1 Antibody (YA4629)

(Synonyms: DCT 1 antibody; dct-1 antibody; DCT1 antibody; Divalent cation transporter 1 antibody; Divalent metal transporter 1 antibody; DMT 1 antibody; DMT-1 antibody; DMT1 antibody; FLJ37416 antibody; Natural resistance associated macrophage protein 2 antibody; Natural resistance-associated macrophage protein 2 antibody; NRAM2_HUMAN antibody; NRAMP 2 antibody; NRAMP2 antibody; OK/SW-cl.20 antibody; Slc11a2 antibody; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody; Solute carrier family 11 member 2 antibody)
Customer Review

Based on 1 Customer Validation

DMT1 Antibody (YA4629) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DMT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • DMT1 Antibody (YA4629) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000 1:1000 1:1000

Product Details

Description

DMT1 Antibody (YA4629) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DMT1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database

Entrez Gene: 4891 Human

SwissProt: P49281 Human

Immunogen

Recombinant protein within human DMT1 aa 1-400.

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719007

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), HepG2 (lane 3(20μg), Hela (lane 4(20μg) using DMT1 (HY-P84933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human prostate tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    DMT1 is a Proton-coupled metal ion symporter operating with a proton to metal ion stoicH2O2etry of 1:1. Selectively transports various divalent metal cations, in decreasing affinity: Cd(2+) > Fe(2+) > Co(2+), Mn(2+) >> Zn(2+), Ni(2+), VO(2+). Essential for maintenance of iron homeostasis by modulating intestinal absorption of dietary Fe(2+) and TF-associated endosomal Fe(2+) transport in erytH2O2d precursors and other cells. Enables Fe(2+) and Mn(2+) ion entry into mitochondria, and is thus expected to promote mitochondrial heme synthesis, iron-sulfur cluster biogenesis and antioxidant defense. Can mediate uncoupled fluxes of either protons or metal ions[1][2][3][4][5][6].

  • Subcellular Localization

    Early endosome membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Extracellular vesicle membrane; Multi-pass membrane protein; Cell membrane; Mitochondrion outer membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Ubiquitously expressed. Expressed in erytH2O2d progenitors

    Induction:Up-regulated under iron-depletion conditions. Down-regulated in response to high extracellular iron levels; Up-regulated under iron-depletion conditions. Down-regulated in response to high extracellular iron levels; Up-regulated under iron-depletion conditions. Down-regulated in response to high extracellular iron levels

  • Isoforms & Post-Translational Modification

    P49281 has 5 isomers: P49281-1: 62266 Da (predicted); P49281-2: 61456 Da (predicted); P49281-3: 64704 Da (predicted); P49281-4: 65514 Da (predicted); P49281-5: 61048 Da (predicted).
    Ubiquitinated by WWP2;N-glycosylated

  • Subunit

    Forms a complex with NDFIP1 and NEDD4L, in cortical neurons, in response to iron and cobalt exposure; this interaction leads to SLC11A2 ubiquitination by NEDD4L and proteasome-dependent degradation (PubMed:19706893).

  • SwissProt ID

    P49281

  • Gene ID
    4891 [NCBI]
  • Synonyms

    DCT 1 antibody; dct-1 antibody; DCT1 antibody; Divalent cation transporter 1 antibody; Divalent metal transporter 1 antibody; DMT 1 antibody; DMT-1 antibody; DMT1 antibody; FLJ37416 antibody; Natural resistance associated macrophage protein 2 antibody; Natural resistance-associated macrophage protein 2 antibody; NRAM2_HUMAN antibody; NRAMP 2 antibody; NRAMP2 antibody; OK/SW-cl.20 antibody; Slc11a2 antibody; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody; Solute carrier family 11 member 2 antibody

DMT1 Antibody (YA4629) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), HepG2 (lane 3(20μg), Hela (lane 4(20μg) using DMT1 (HY-P84933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    1/16
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • DMT1 Antibody (YA4629) Product Image
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), HepG2 (lane 3(20μg), Hela (lane 4(20μg) using DMT1 (HY-P84933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • Experimental Validation Results for DMT1 Antibody (YA4629)
  • DMT1 Antibody (YA4629) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), HepG2 (lane 3(20μg), Hela (lane 4(20μg) using DMT1 (HY-P84933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using DMT1 Antibody (HY-P84933, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DMT1 Antibody (YA4629) Product Image WB
  • DMT1 Antibody (YA4629) Product Image IHC-P
  • DMT1 Antibody (YA4629) Product Image IHC-P
  • DMT1 Antibody (YA4629) Product Image IHC-P
  • DMT1 Antibody (YA4629) Product Image IHC-P
  • DMT1 Antibody (YA4629) Product Image IHC-P
  • DMT1 Antibody (YA4629) Product Image IHC-P
  • DMT1 Antibody (YA4629) Product Image IHC-P