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ID1 Antibody (YA2845)

(Synonyms: DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; ID1; BHLHB24; ID)
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Based on 1 Customer Validation

ID1 Antibody (YA2845) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ID1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ID1 Antibody (YA2845)
  • ID1 Antibody (YA2845) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

ID1 Antibody (YA2845) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ID1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 16 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Id1 aa1-63.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105216

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ID1 Antibody (YA2845)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), K562 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and MDA-MB-231 (lane 6, 15 μg) using ID1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ID1 transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    P41134 has 2 isomers: P41134-1: 16133 Da (predicted); P41134-2: 15586 Da (predicted).

  • Subunit

    Heterodimer with other HLH proteins. Interacts with COPS5, IFI204, GATA4 and NKX2-5 (By similarity). Interacts with CLOCK and BMAL1

  • SwissProt ID

    P41134

  • Gene ID
    3397 [NCBI]
  • Synonyms

    DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; ID1; BHLHB24; ID

  • Research Field

    Epigenetics and Nuclear Signaling

ID1 Antibody (YA2845) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), K562 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and MDA-MB-231 (lane 6, 15 μg) using ID1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ID1 Antibody (YA2845) Product Image
    1/16
  • Experimental Validation Results for ID1 Antibody (YA2845)
  • ID1 Antibody (YA2845) Product Image
  • Experimental Validation Results for ID1 Antibody (YA2845)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), K562 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and MDA-MB-231 (lane 6, 15 μg) using ID1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ID1 Antibody (YA2845)
  • ID1 Antibody (YA2845) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), K562 (lane 4, 15 μg), Jurkat (lane 5, 15 μg), and MDA-MB-231 (lane 6, 15 μg) using ID1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ID1 Antibody (YA2845) Product Image WB