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ALDH7A1 Antibody (YA2034)(PBS only)

(Synonyms: aldehyde dehydrogenase 7 family member A1; EPD; PDE; ATQ1)

ALDH7A1 Antibody (YA2034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDH7A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)(PBS only)
  • ALDH7A1 Antibody (YA2034)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

ALDH7A1 Antibody (YA2034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDH7A1.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Immunogen

A synthetic peptide of human ALDH7A1 aa16-29.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)(PBS only)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ALDH7A1 multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus; Mitochondrion

  • Expression


    Tissue_specificity:Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in human peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue

  • Isoforms & Post-Translational Modification

    P49419 has 3 isomers: P49419-1: 58487 Da (predicted); P49419-2: 55366 Da (predicted); P49419-4: 51411 Da (predicted).

  • Subunit

    Homotetramer

  • SwissProt ID

    P49419

  • Gene ID
    501 [NCBI]
  • Synonyms

    aldehyde dehydrogenase 7 family member A1; EPD; PDE; ATQ1

  • Research Field

    Signal Transduction

ALDH7A1 Antibody (YA2034)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ALDH7A1 Antibody (YA2034)(PBS only) Product Image
    1/16
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)(PBS only)
  • ALDH7A1 Antibody (YA2034)(PBS only) Product Image
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)(PBS only)
  • ALDH7A1 Antibody (YA2034)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ALDH7A1 Antibody (YA2034)(PBS only) Product Image WB