Phospho-Rb2 p130 (Thr986) Antibody (YA1035)(PBS only)
(Synonyms: Rb2; P130)Phospho-Rb2 p130 (Thr986) Antibody (YA1035) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Rb2 p130 (Thr986).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Phospho-Rb2 p130 (Thr986) Antibody (YA1035) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Rb2 p130 (Thr986).
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 128 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 128 kDa
A synthetic phosphopeptide corresponding to residues surrounding Thr986 of human p130
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Rb2 p130 is key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
Q08999 has 2 isomers: Q08999-1: 128367 Da (predicted); Q08999-2: 59349 Da (predicted).
During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis -
Subunit
Interacts with AATF. Interacts with KMT5B, KMT5C and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with RBBP9 (By similarity). Interacts with CD53 (By similarity)
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SwissProt ID
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Synonyms
Rb2; P130
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Research Field
Cell Biology
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