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Phospholipase C gamma 1 Antibody (YA179)

(Synonyms: PLC gamma 1 antibody; PLC II antibody; PLC-148 antibody; PLCgamma1 antibody; PLCG1; NCKAP3 )
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Based on 1 Customer Validation

Phospholipase C gamma 1 Antibody (YA179) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C gamma 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
  • Phospholipase C gamma 1 Antibody (YA179) Product Image
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

Phospholipase C gamma 1 Antibody (YA179) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C gamma 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 149 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 149 kDa
Species Reactivity Database

Entrez Gene: 5335 Human

SwissProt: P19174 Human

Immunogen

Synthetic peptide corresponding to Human Phospholipase C gamma 1.AA range:690-852.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102510

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), 293T (lane 4), HeLa (lane 5) and U87 (lane 6) using Phospholipase C gamma 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
    Western blot analysis of extracts from RAW264.7(lane 2(10μg) or lane 3(20μg) ) and NIH3T3 (lane 4(10μg) or lane 5(20μg) ) using Phospholipase C gamma 1(HY-P80427) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80427, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Phospholipase C gamma 1 mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis[1].

  • Subcellular Localization

    Cell projection, lamellipodium; Cell projection, ruffle

  • Isoforms & Post-Translational Modification

    P19174 has 2 isomers: P19174-1: 148532 Da (predicted); P19174-2: 148660 Da (predicted).
    Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA (By similarity). Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells;Ubiquitinated by CBLB in activated T-cells

  • Subunit

    Interacts with AGAP2 via its SH3 domain. Interacts (via SH2 domain) with RET. Interacts with FLT1 (tyrosine-phosphorylated) (By similarity). Interacts (via SH2 domain) with FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with LAT (phosphorylated) upon TCR activation. Interacts (via SH3 domain) with the Pro-rich domain of TNK1. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts (via SH3 domain) with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK (By similarity). Interacts with AXL, FLT4 and KIT. Interacts with RALGPS1. Interacts (via the SH2 domains) with VIL1 (phosphorylated at C-terminus tyrosine phosphorylation sites). Interacts (via SH2 domain) with PDGFRA and PDGFRB (tyrosine phosphorylated). Interacts with PIP5K1C (By similarity). Interacts with NTRK1 and NTRK2 (phosphorylated upon ligand-binding). Interacts with SYK; activates PLCG1. Interacts with GRB2, LAT and THEMIS upon TCR activation in thymocytes (By similarity). Interacts with TESPA1; the association is increased with prolonged stimulation of the TCR and may facilitate the assembly of the LAT signalosome. Interacts (via C-terminal proline-rich domain (PRD)) with PLCG1 (via SH3 domain); this interaction leads to guanine nucleotide exchange from PlCG1 to DNM1 and enhances DNM1-dependent endocytosis (By similarity)

  • SwissProt ID

    P19174

  • Gene ID
    5335 [NCBI]
  • Synonyms

    PLC gamma 1 antibody; PLC II antibody; PLC-148 antibody; PLCgamma1 antibody; PLCG1; NCKAP3

  • Research Field

    Cardiovascular

[1]. Wang Y, et al. Obligatory role for phospholipase C-gamma(1) in villin-induced epithelial cell migration. Am J Physiol Cell Physiol. 2007 May;292(5):C1775-86. [Content Brief]

Phospholipase C gamma 1 Antibody (YA179) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), 293T (lane 4), HeLa (lane 5) and U87 (lane 6) using Phospholipase C gamma 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(10μg) or lane 3(20μg) ) and NIH3T3 (lane 4(10μg) or lane 5(20μg) ) using Phospholipase C gamma 1(HY-P80427) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80427, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Phospholipase C gamma 1 Antibody (YA179) Product Image
    1/16
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
  • Phospholipase C gamma 1 Antibody (YA179) Product Image
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), 293T (lane 4), HeLa (lane 5) and U87 (lane 6) using Phospholipase C gamma 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(10μg) or lane 3(20μg) ) and NIH3T3 (lane 4(10μg) or lane 5(20μg) ) using Phospholipase C gamma 1(HY-P80427) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80427, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
  • Experimental Validation Results for Phospholipase C gamma 1 Antibody (YA179)
  • Phospholipase C gamma 1 Antibody (YA179) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), MCF-7 (lane 3), 293T (lane 4), HeLa (lane 5) and U87 (lane 6) using Phospholipase C gamma 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Phospholipase C gamma 1 Antibody (YA179) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(10μg) or lane 3(20μg) ) and NIH3T3 (lane 4(10μg) or lane 5(20μg) ) using Phospholipase C gamma 1(HY-P80427) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80427, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Phospholipase C gamma 1 Antibody (YA179) Product Image WB
  • Phospholipase C gamma 1 Antibody (YA179) Product Image WB