CSDE1 Antibody (YA2828)(PBS only)
(Synonyms: UNR; D1S155E)CSDE1 Antibody (YA2828) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CSDE1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 | 1:50-1:200 | 1:20 |
Product Details
CSDE1 Antibody (YA2828) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CSDE1.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 89 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 89 kDa
A synthetic peptide of human CSDE1/NRU
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from RAW264.7 (lane 2(20μg) , Hela (lane 3(20μg) , A431 (lane 4(20μg),using CSDE1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling CSDE1 with CSDE1 Antibody (HY-P83083) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CSDE1 Antibody (HY-P83083) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of MCF-7 cells labeling CSDE1 with CSDE1 Antibody (HY-P83083) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CSDE1 Antibody (HY-P83083) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
CSDE1 is a RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules; (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA[1][2][3].
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Subcellular Localization
Cytoplasm; Cytoplasm, Stress granule; Cytoplasm, P-body
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Isoforms & Post-Translational Modification
O75534 has 5 isomers: O75534-1: 88885 Da (predicted); O75534-2: 85747 Da (predicted); O75534-3: 90605 Da (predicted); O75534-4: 93742 Da (predicted); O75534-5: 69459 Da (predicted).
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Subunit
Component of a multi subunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1 (PubMed:16356927). Interacts with STRAP (PubMed:10049359). Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, HNRPD and SYNCRIP (PubMed:11051545). The interaction with PABPC1 is direct and RNA-independent (PubMed:11051545, PubMed:15314026). Interacts with EIF4ENIF1/4E-T (PubMed:27342281, PubMed:32354837)
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SwissProt ID
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Synonyms
UNR; D1S155E
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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