p150 CAF1 Antibody (YA1488)(PBS only)
(Synonyms: CHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150)p150 CAF1 Antibody (YA1488) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p150 CAF1.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF, FC
-
Reactivity :
Human
-
Formulation:
Supplied in PBS, pH 7.4.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:100-1:200 | 1:20-1:50 | 1:20-1:50 |
Product Details
p150 CAF1 Antibody (YA1488) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p150 CAF1.
-
Host Rabbit
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 150 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 107 kDa
A synthetic peptide of human p150 CAF1
Affinity Purified
Non-conjugated
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS, pH 7.4.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
p150 CAF1 acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci
-
Subcellular Localization
Nucleus
-
Isoforms & Post-Translational Modification
Q13111 has 3 isomers: Q13111-1: 106910 Da (predicted); Q13111-2: 84685 Da (predicted); Q13111-3: 88269 Da (predicted).
-
Subunit
Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Interacts with PCNA; the interaction is direct (PubMed:10052459, PubMed:10648606). Interacts (via the PxVxL motif) with CBX5; the interaction is direct (PubMed:15882967, PubMed:20562864). Interacts with MBD1 (PubMed:12697822). Interacts with histones H3.1, H3.2 and H3.1t (PubMed:33857403)
-
SwissProt ID
-
Synonyms
CHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150
-
Research Field
Epigenetics and Nuclear Signaling
Documentation
Powered by Bioz
