C4A Antibody (YA1505)
(Synonyms: Acidic C4; Acidic complement C4; Basic C4; Basic complement C4; C4A; C4A2; C4A3; C4A4; C4A6; C4AD; C4B1; C4B12; C4B2; C4B3; C4BD; C4F; C4S; CPAMD2; CPAMD3)C4A Antibody (YA1505) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C4A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 |
Product Details
C4A Antibody (YA1505) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C4A.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 193 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 193 kDa
A synthesized peptide derived from human C4D aa940-1237.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
C4A is a Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system; Non-enzymatic component of C3 and C5 convertases. Generated following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway), it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal. It then recruits the serine protease complement C2b to form the C3 and C5 convertases, which cleave and activate C3 and C5, respectively, the next components of the complement pathways. Complement C4b-A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while complement C4b-B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens; Putative humoral mediator released following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway). While it is strongly similar to anaphylatoxins, its role is unclear. Was reported to act as a mediator of local inflammatory process; however these effects were probably due to contamination with C3a and/C5a anaphylatoxins in biological assays[1][2][3][4][5][6][7][8].
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Subcellular Localization
Secreted; Synapse; Cell projection, axon; Cell projection, dendrite; Secreted; Secreted; Cell surface
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Expression
Tissue_specificity:Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen (PubMed:11367523) . The extra-hepatic sites of expression may be important for the local protection and inflammatory response (PubMed:11367523) -
Isoforms & Post-Translational Modification
P0C0L4 has 2 isomers: P0C0L4-1: 192785 Da (predicted); P0C0L4-2: 187704 Da (predicted).
Prior to secretion, the single-chain precursor is enzymatically cleaved by plasminogen (PLG) to yield non-identical chains alpha, beta and gamma (PubMed:76991). During activation of the complement systems, the alpha chain is cleaved into C4a and C4b by different proteases depending on the complement pathway: C4b stays linked to the beta and gamma chains, while C4a is released in the plasma (PubMed:11527969, PubMed:16169853, PubMed:22949645, PubMed:467643, PubMed:6319179, PubMed:9422791). The alpha chain is cleaved by C1S to generate C4a and C4b following activation by the classical complement system (PubMed:11527969, PubMed:16169853, PubMed:22949645, PubMed:467643, PubMed:6319179, PubMed:9422791). The alpha chain is cleaved to generate C4a and C4b by MASP2 following activation by the lectin complement system (PubMed:11527969, PubMed:22949645). The alpha chain is cleaved by GZMK to generate C4a and C4b following activation by the GZMK complement system (PubMed:39914456). Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase (PubMed:3696167). The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma (PubMed:3696167);Upon activation, the internal thioester bond reacts with carbohydrate antigens on the target surface to form amide or ester bonds, leading to covalent association with the surface of pathogens;Ser-1236 of complement C4b interacts with complement C3b via a thioester linkage;N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan -
Subunit
Complement circulates in blood as a disulfide-linked trimer of an alpha, beta and gamma chain
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SwissProt ID
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Synonyms
Acidic C4; Acidic complement C4; Basic C4; Basic complement C4; C4A; C4A2; C4A3; C4A4; C4A6; C4AD; C4B1; C4B12; C4B2; C4B3; C4BD; C4F; C4S; CPAMD2; CPAMD3
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Research Field
Immunology
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