CD177 Antibody (YA4371)(PBS only)
(Synonyms: NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP)CD177 Antibody (YA4371) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD177.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
CD177 Antibody (YA4371) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD177.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 100-130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 46 kDa
Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD177 is an In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils. By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis[1][2][3][4][5][6][7].
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Subcellular Localization
Cell membrane; Lipid-anchor, GPI-anchor; Membrane raft; Lipid-anchor, GPI-like-anchor; Secreted; Cytoplasmic granule membrane; Cell projection, lamellipodium
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Expression
Tissue_specificity:Highly expressed in normal bone marrow and weakly expressed in fetal liver (PubMed:10753836) . During neutrophil differentiation, expression begins at the metamyelocyte stage and continues tH2O2ghout the subsequent stages (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:24926686) . Expressed by a subset of mature neutrophils (at protein level) (PubMed:10753836, PubMed:12377969, PubMed:12675722, PubMed:17244676, PubMed:17580308, PubMed:18462208, PubMed:21193407, PubMed:24926686, PubMed:27227454, PubMed:28240246, PubMed:28807980) . The percentage of neutrophils expressing CD177 varies across the population (PubMed:17244676, PubMed:27227454) . Expressed in granulocytes of with polycythemia vera (PV) and with essential tH2O2bocythemia (ET) (PubMed:10753836, PubMed:12377969)
Induction:By CSF3/G-CSF in resting granulocytes (PubMed:10753836) . Induced during CSF3/G-CSF-mediated neutrophil differentiation (PubMed:17244676, PubMed:18462208) . Induced during pregnancy (PubMed:12675722) . Induced with polycythemia vera (PV) and with essential tH2O2bocythemia (ET) (PubMed:10753836, PubMed:12377969) -
Isoforms & Post-Translational Modification
Q8N6Q3 has 3 isomers: Q8N6Q3-1: 46363 Da (predicted); Q8N6Q3-2: 27061 Da (predicted); Q8N6Q3-3: 15683 Da (predicted).
N-glycosylated;A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding) -
Subunit
Found in a complex with integrin ITGAM/CD11b and ITGB2/CD18 (PubMed:21193407, PubMed:28807980). Interacts with PECAM1 (via Ig-like C2-type domain 6); the interaction is Ca(2+)-dependent; the interaction is direct (PubMed:17580308). Interacts with serine protease PRTN3/myeloblastin; the interaction tethers PRTN3 to the cell surface; the interaction is direct (PubMed:17244676, PubMed:28240246)
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SwissProt ID
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Synonyms
NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
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