CD177 Antibody (YA4371)(PBS only)

Cat. No.: HY-P84674A: Data Sheet User Guide for Antibodies
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CD177 Antibody (YA4371) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD177.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD177 Antibody (YA4371) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD177.

Host

Mouse

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 100-130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8N6Q3

Gene ID

57126 [NCBI]

Immunogen

Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369)

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Membrane raft; Lipid-anchor, GPI-like-anchor; Secreted; Cytoplasmic granule membrane; Cell projection, lamellipodium

Expression：
Tissue_specificity:Highly expressed in normal bone marrow and weakly expressed in fetal liver (PubMed:10753836) . During neutrophil differentiation, expression begins at the metamyelocyte stage and continues tH2O2ghout the subsequent stages (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:24926686) . Expressed by a subset of mature neutrophils (at protein level) (PubMed:10753836, PubMed:12377969, PubMed:12675722, PubMed:17244676, PubMed:17580308, PubMed:18462208, PubMed:21193407, PubMed:24926686, PubMed:27227454, PubMed:28240246, PubMed:28807980) . The percentage of neutrophils expressing CD177 varies across the population (PubMed:17244676, PubMed:27227454) . Expressed in granulocytes of with polycythemia vera (PV) and with essential tH2O2bocythemia (ET) (PubMed:10753836, PubMed:12377969)

Induction:By CSF3/G-CSF in resting granulocytes (PubMed:10753836) . Induced during CSF3/G-CSF-mediated neutrophil differentiation (PubMed:17244676, PubMed:18462208) . Induced during pregnancy (PubMed:12675722) . Induced with polycythemia vera (PV) and with essential tH2O2bocythemia (ET) (PubMed:10753836, PubMed:12377969)

Isoforms & Post-Translational Modification：Q8N6Q3 has 3 isomers: Q8N6Q3-1: 46363 Da (predicted); Q8N6Q3-2: 27061 Da (predicted); Q8N6Q3-3: 15683 Da (predicted).
N-glycosylated;A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding)

Subunit：Found in a complex with integrin ITGAM/CD11b and ITGB2/CD18 (PubMed:21193407, PubMed:28807980). Interacts with PECAM1 (via Ig-like C2-type domain 6); the interaction is Ca(2+)-dependent; the interaction is direct (PubMed:17580308). Interacts with serine protease PRTN3/myeloblastin; the interaction tethers PRTN3 to the cell surface; the interaction is direct (PubMed:17244676, PubMed:28240246)

Synonyms

NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP

Documentation

User Guide for Antibodies (1077 KB)