MAP4K4/NIK Antibody (YA4892)

Cat. No.: HY-P85200: Data Sheet User Guide for Antibodies
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MAP4K4/NIK Antibody (YA4892) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MAP4K4/NIK.

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사이즈	가격	재고
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

MAP4K4/NIK Antibody (YA4892) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MAP4K4/NIK.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 142 kDa;

Species Reactivity

Human, Mouse

SwissProt ID

O95819

Gene ID

9448 [NCBI]

Immunogen

Purified recombinant fragment of HGK (aa400-500) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1/200 - 1/400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in ascitic fluid containing 0.03% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway (PubMed:9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). Phosphorylates SMAD1 on Thr-322 (PubMed:21690388)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Widely expressed. Isomer 5 is abundant in the brain. Isomer 4 is mainly found in the liver, skeletal muscle, and placenta.

Isoforms & Post-Translational Modification：O95819 has 6 isomers: O95819-1: 142101 Da (predicted); O95819-2: 138505 Da (predicted); O95819-3: 151000 Da (predicted); O95819-4: 133401 Da (predicted); O95819-5: 141929 Da (predicted); O95819-6: 145005 Da (predicted).

Subunit：Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially)

Synonyms

MAP4K4; HGK; KIAA0687; NIK; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase

각종 서류

User Guide for Antibodies (1077 KB)