MAP4K4/NIK Antibody (YA4892)
(Synonyms: MAP4K4; HGK; KIAA0687; NIK; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase)MAP4K4/NIK Antibody (YA4892) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MAP4K4/NIK.
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Host:
Mouse
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Application:
WB, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in ascitic fluid containing 0.03% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1/200 - 1/400 | 1:10000 |
Product Details
MAP4K4/NIK Antibody (YA4892) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to MAP4K4/NIK.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Calculated Molecular Weight Predicted band size: 142 kDa;
Purified recombinant fragment of HGK (aa400-500) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in ascitic fluid containing 0.03% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MAP4K4/NIK is a Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway. Phosphorylates SMAD1 on Thr-322[1][2][3].
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Subcellular Localization
Cytoplasm
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Expression
Tissue_specificity:Widely expressed. Isomer 5 is abundant in the brain. Isomer 4 is mainly found in the liver, skeletal muscle, and placenta. -
Isoforms & Post-Translational Modification
O95819 has 6 isomers: O95819-1: 142101 Da (predicted); O95819-2: 138505 Da (predicted); O95819-3: 151000 Da (predicted); O95819-4: 133401 Da (predicted); O95819-5: 141929 Da (predicted); O95819-6: 145005 Da (predicted).
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Subunit
Interacts with the SH3 domain of the adapter proteins Nck (By similarity). Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially)
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SwissProt ID
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Synonyms
MAP4K4; HGK; KIAA0687; NIK; Mitogen-activated protein kinase kinase kinase kinase 4; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase
Documentation
References
[1]. Yao Z, et al. A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway. J Biol Chem. 1999 Jan 22;274(4):2118-25. [Content Brief]
[2]. Meng Z, et al. MAP4K family kinases act in parallel to MST1/2 to activate LATS1/2 in the Hippo pathway. Nat Commun. 2015 Oct 5;6:8357. [Content Brief]
[3]. Kaneko S, et al. Smad inhibition by the Ste20 kinase Misshapen. Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):11127-32. [Content Brief]
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