2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Legumain Antibody (YA2469)

Legumain Antibody (YA2469)

Cat. No.: HY-P82724
COA
SDS
User Guide for Antibodies Technical Support

Legumain Antibody (YA2469) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Legumain.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

Legumain Antibody (YA2469) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Legumain.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 49 kDa;
Observed band size: 36,57 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Legumain aa200-350.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine Legumain Antibody (HY-P82724) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed:9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity)
Subcellular Localization：Lysosome
Expression：
Tissue_specificity:Ubiquitous. Particularly abundant in kidney, heart and placenta
Isoforms & Post-Translational Modification：Q99538 has 3 isomers: Q99538-1: 49411 Da (predicted); Q99538-2: 42948 Da (predicted); Q99538-3: 42008 Da (predicted).
Activated by autocatalytic processing at pH 4
Subunit：Homodimer before autocatalytic removal of the propeptide (PubMed:23776206). Monomer after autocatalytic processing (PubMed:23776206). May interact with integrins (PubMed:23776206)
RRID
Database

Entrez Gene: 5641 Human ; 19141 Mouse ; 63865 Rat

SwissProt: Q99538 Human ; O89017 Mouse ; Q9R0J8 Rat

OMIM: 602620 Human

Research Field

Immunology
Synonyms
AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Legumain Antibody (YA2469) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Legumain Antibody (YA2469)
Cat. No.:
HY-P82724
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