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Legumain Antibody (YA2469)

(Synonyms: AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1)
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Based on 1 Customer Validation

Legumain Antibody (YA2469) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Legumain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Legumain Antibody (YA2469)
  • Legumain Antibody (YA2469) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Legumain Antibody (YA2469) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Legumain.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36,57 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Legumain aa200-350.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104822

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Legumain Antibody (YA2469)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine Legumain Antibody (HY-P82724) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Legumain is also known as asparaginyl endopeptidase or δ-secretase. Legumain is a family of cysteine proteases that specifically cleave peptide bonds at their carboxyl-terminal side of Asn or Asp residues. In plants, Legumains are key enzymes in the processing and activation of seed storage proteins during seed maturation. In mammals, Legumain mainly plays its protease activity. Legumain facilitates haemoglobin digestion. Legumain is also involved in processing self and foreign proteins for presentation on the MHCII complex. Besides, Legumain takes part in osteoclast remodeling. Legumain has broad tissue distribution, and is highly expressed in the kidneys, placenta, spleen, bladder, lungs, and thyroid and lymph nodes. Legumain is also overexpressed in cancers and promotes cancer cell migration[1][2].

  • Subcellular Localization

    Lysosome

  • Expression


    Tissue_specificity:Ubiquitous. Particularly abundant in kidney, heart and placenta

  • Isoforms & Post-Translational Modification

    Q99538 has 3 isomers: Q99538-1: 49411 Da (predicted); Q99538-2: 42948 Da (predicted); Q99538-3: 42008 Da (predicted).
    Activated by autocatalytic processing at pH 4

  • Subunit

    Homodimer before autocatalytic removal of the propeptide (PubMed:23776206). Monomer after autocatalytic processing (PubMed:23776206). May interact with integrins (PubMed:23776206)

  • SwissProt ID

    Q99538

  • Gene ID
    5641 [NCBI]
  • Synonyms

    AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1

  • Research Field

    Immunology

References

[1]. Song M. The Asparaginyl Endopeptidase Legumain: An Emerging Therapeutic Target and Potential Biomarker for Alzheimer's Disease. Int J Mol Sci. 2022 Sep 6;23(18):10223. [Content Brief]

[2]. Dall E, et al. Structure and function of legumain in health and disease. Biochimie. 2016 Mar;122:126-50. [Content Brief]

Legumain Antibody (YA2469) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine Legumain Antibody (HY-P82724) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Legumain Antibody (YA2469) Product Image
    1/16
  • Experimental Validation Results for Legumain Antibody (YA2469)
  • Legumain Antibody (YA2469) Product Image
  • Experimental Validation Results for Legumain Antibody (YA2469)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine Legumain Antibody (HY-P82724) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Legumain Antibody (YA2469)
  • Legumain Antibody (YA2469) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Monoamine Legumain Antibody (HY-P82724) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Legumain Antibody (YA2469) Product Image WB