PABP Antibody (YA6696)

Cat. No.: HY-P87003: Data Sheet COA
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PABP Antibody (YA6696) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PABP.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

PABP Antibody (YA6696) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PABP.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 71 kDa;

Observed band size: 71 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

P11940

Gene ID

26986 [NCBI]

Immunogen

Recombinant protein within Human PABP protein aa 450-550.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
FC FC: Flow Cytometry	1:1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C2C12 (lane 4(20μg)) using PABP Antibody (HY-P87003) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299). Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744). Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Involved in translationally coupled mRNA turnover (PubMed:11051545). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545). Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299)|(Microbial infection) Positively regulates the replication of dengue virus (DENV)

Subcellular Localization：Cytoplasm,Cytoplasm, Stress granule,Nucleus,Cell projection, lamellipodium

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：P11940 has two isomers: P11940-1: 70671 Da (predicted); P11940-2: 61181 Da (predicted).
Phosphorylated by MAPKAPK2丨Methylated by CARM1

Subunit：May form homodimers

RRID

AB_3719244

Synonyms

PAB 1 antibody; PAB1 antibody; PABP 1 antibody; PABP-1 antibody; PABP1 antibody; PABP1_HUMAN antibody; PABPC 1 antibody; Pabpc1 antibody; PABPC2 antibody; PABPL1 antibody; PAB 1 antibody; PAB1 antibody; PABP 1 antibody; PABP-1 antibody; PABP1 antibody; PABP1_HUMAN antibody; PABPC 1 antibody; Pabpc1 antibody; PABPC2 antibody; PABPL1 antibody; Poly A binding protein 1 antibody; Poly A binding protein cytoplasmic 1 antibody; poly(A) binding protein, cytoplasmic 1 antibody; poly(A) binding protein, cytoplasmic 2 antibody; Poly(A)-binding protein 1 antibody; Polyadenylate binding protein 1 antibody; Polyadenylate-binding protein 1 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)