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PABP Antibody (YA6696)

(Synonyms: PAB 1 antibody; PAB1 antibody; PABP 1 antibody; PABP-1 antibody; PABP1 antibody; PABP1_HUMAN antibody; PABPC 1 antibody; Pabpc1 antibody; PABPC2 antibody; PABPL1 antibody; PAB 1 antibody; PAB1 antibody; PABP 1 antibody; PABP-1 antibody; PABP1 antibody; PABP1_HUMAN antibody; PABPC 1 antibody; Pabpc1 antibody; PABPC2 antibody; PABPL1 antibody; Poly A binding protein 1 antibody; Poly A binding protein cytoplasmic 1 antibody; poly(A) binding protein, cytoplasmic 1 antibody; poly(A) binding protein, cytoplasmic 2 antibody; Poly(A)-binding protein 1 antibody; Polyadenylate binding protein 1 antibody; Polyadenylate-binding protein 1 antibody; )
Customer Review

Based on 1 Customer Validation

PABP Antibody (YA6696) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PABP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PABP Antibody (YA6696)
  • PABP Antibody (YA6696) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:1000 1:100 1:1000 1:1000 1:200

Product Details

Description

PABP Antibody (YA6696) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PABP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey
  • Observed Molecular Weight
    Observed band size: 71 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 71 kDa
Immunogen

Recombinant protein within Human PABP protein aa 450-550.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719244

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PABP Antibody (YA6696)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C2C12 (lane 4(20μg)) using PABP Antibody (HY-P87003) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PABP binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability |(Microbial infection) Positively regulates the replication of dengue virus (DENV)[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm,Cytoplasm, Stress granule,Nucleus,Cell projection, lamellipodium

  • Expression


    Tissue_Specificity: Ubiquitous

  • Isoforms & Post-Translational Modification

    P11940 has two isomers: P11940-1: 70671 Da (predicted); P11940-2: 61181 Da (predicted).
    Phosphorylated by MAPKAPK2丨Methylated by CARM1

  • Subunit

    May form homodimers

  • SwissProt ID

    P11940

  • Gene ID
    26986 [NCBI]
  • Synonyms

    PAB 1 antibody; PAB1 antibody; PABP 1 antibody; PABP-1 antibody; PABP1 antibody; PABP1_HUMAN antibody; PABPC 1 antibody; Pabpc1 antibody; PABPC2 antibody; PABPL1 antibody; PAB 1 antibody; PAB1 antibody; PABP 1 antibody; PABP-1 antibody; PABP1 antibody; PABP1_HUMAN antibody; PABPC 1 antibody; Pabpc1 antibody; PABPC2 antibody; PABPL1 antibody; Poly A binding protein 1 antibody; Poly A binding protein cytoplasmic 1 antibody; poly(A) binding protein, cytoplasmic 1 antibody; poly(A) binding protein, cytoplasmic 2 antibody; Poly(A)-binding protein 1 antibody; Polyadenylate binding protein 1 antibody; Polyadenylate-binding protein 1 antibody;

PABP Antibody (YA6696) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C2C12 (lane 4(20μg)) using PABP Antibody (HY-P87003) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PABP Antibody (YA6696) Product Image
    1/16
  • Experimental Validation Results for PABP Antibody (YA6696)
  • PABP Antibody (YA6696) Product Image
  • Experimental Validation Results for PABP Antibody (YA6696)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C2C12 (lane 4(20μg)) using PABP Antibody (HY-P87003) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PABP Antibody (YA6696)
  • PABP Antibody (YA6696) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C2C12 (lane 4(20μg)) using PABP Antibody (HY-P87003) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PABP Antibody (YA6696) Product Image WB