p47 phox Antibody
(Synonyms: Neutrophil cytosol factor 1, NCF-1, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, Ncf1)p47 phox Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to p47 phox.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
p47 phox Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to p47 phox.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
Synthetic peptide corresponding to the C-term region of human p47 phox.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
p47 phox is a Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox[1][2][3][4][5].
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Subcellular Localization
Cytoplasm, cytosol; Membrane; Peripheral membrane protein; Cytoplasmic side
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Expression
Tissue_specificity:Detected in peripheral blood monocytes and neutrophils (at protein level) -
Isoforms & Post-Translational Modification
P14598 has 2 isomers: P14598-1: 44682 Da (predicted); P14598-2: 30811 Da (predicted).
Phosphorylated by PRKCD; phosphorylation induces activation of NCF1, leading to assembly and activation of the NADPH oxidase complex -
Subunit
Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2 (PubMed:38355798). Part of a cytosolic complex composed at least by NCF1, NCF2 and NCF4 (PubMed:8280052). Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain) (PubMed:12169629). Interacts with NCF4 (PubMed:15657040). Interacts with CYBB (PubMed:9224653). Interacts (via the second SH3 domain) with CYBA; interaction is phosphorylation-dependent (PubMed:12732142, PubMed:16326715). Interacts with NOXA1 (PubMed:12716910). Interacts with ADAM15 (PubMed:19718658). Interacts with TRAF4 (PubMed:12023963, PubMed:16052631). Interacts with FASLG (PubMed:19807924). Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity)
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SwissProt ID
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Synonyms
Neutrophil cytosol factor 1, NCF-1, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, Ncf1
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