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MDH1 Antibody (YA2082)

(Synonyms: MDH s; mdh1; MDHA; MDHs; MOR2)
Customer Review

Based on 1 Customer Validation

MDH1 Antibody (YA2082) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MDH1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC FC
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • MDH1 Antibody (YA2082) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50 1:50-1:100

Product Details

Description

MDH1 Antibody (YA2082) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MDH1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MDH1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104446

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MDH1 Antibody (YA2082)
    Western blot analysis of extracts from Hela (lane 2(20μg)), Jurkat (lane 2(20μg)) and HepG2 (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    Western blot analysis of extracts from Mouse liver (lane 2(20μg)) and Rat liver (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    Flow cytometric analysis of 1X106 NIH-3T3 cells labeling MDH1 Antibody(HY-P82337, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MDH1 catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS)[1][2][3][4].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Isoforms & Post-Translational Modification

    P40925 has 3 isomers: P40925-1: 36426 Da (predicted); P40925-2: 27025 Da (predicted); P40925-3: 38628 Da (predicted).
    ISGylated;Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation

  • Subunit

    Homodimer

  • SwissProt ID

    P40925

  • Gene ID
    4190 [NCBI]
  • Synonyms

    MDH s; mdh1; MDHA; MDHs; MOR2

  • Research Field

    Signal Transduction

MDH1 Antibody (YA2082) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)), Jurkat (lane 2(20μg)) and HepG2 (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Mouse liver (lane 2(20μg)) and Rat liver (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH-3T3 cells labeling MDH1 Antibody(HY-P82337, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MDH1 Antibody (YA2082) Product Image
    1/16
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • MDH1 Antibody (YA2082) Product Image
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)), Jurkat (lane 2(20μg)) and HepG2 (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Mouse liver (lane 2(20μg)) and Rat liver (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MDH1 Antibody (YA2082)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH-3T3 cells labeling MDH1 Antibody(HY-P82337, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • Experimental Validation Results for MDH1 Antibody (YA2082)
  • MDH1 Antibody (YA2082) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg)), Jurkat (lane 2(20μg)) and HepG2 (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MDH1 Antibody (YA2082) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Mouse liver (lane 2(20μg)) and Rat liver (lane 2(20μg)) using MDH1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MDH1 Antibody (YA2082) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MDH1 Antibody (YA2082) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MDH1 with MDH1 Antibody (HY-P82337) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MDH1 Antibody (HY-P82337) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MDH1 Antibody (YA2082) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 NIH-3T3 cells labeling MDH1 Antibody(HY-P82337, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MDH1 Antibody (YA2082) Product Image WB
  • MDH1 Antibody (YA2082) Product Image WB
  • MDH1 Antibody (YA2082) Product Image ICC
  • MDH1 Antibody (YA2082) Product Image ICC
  • MDH1 Antibody (YA2082) Product Image FC