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Mitochondrial Ferritin Antibody (YA3353)

(Synonyms: FerH; Ferritin; Ferritin H subunit; Ferritin; mitochondrial; Fth3; FTMT; mitochondrial; Mitochondrial ferritin; MTF)
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Based on 1 Customer Validation

Mitochondrial Ferritin Antibody (YA3353) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mitochondrial Ferritin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Mitochondrial Ferritin Antibody (YA3353)
  • Mitochondrial Ferritin Antibody (YA3353) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Mitochondrial Ferritin Antibody (YA3353) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mitochondrial Ferritin.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database

Entrez Gene: 94033 Human

SwissProt: Q8N4E7 Human

Immunogen

A synthesized peptide derived from human FTMT aa200-242/242.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105715

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Mitochondrial Ferritin Antibody (YA3353)
    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using Mitochondrial Ferritin Antibody (HY-P83608) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Mitochondrial Ferritin catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation[1][2].

  • Subcellular Localization

    Mitochondrion

  • Expression


    Tissue_specificity:Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine

  • Subunit

    Homooligomer of 24 subunits. The functional molecule is roughly spherical and contains a central cavity into which the polymeric mineral iron core is deposited

  • SwissProt ID

    Q8N4E7

  • Gene ID
    94033 [NCBI]
  • Synonyms

    FerH; Ferritin; Ferritin H subunit; Ferritin; mitochondrial; Fth3; FTMT; mitochondrial; Mitochondrial ferritin; MTF

  • Research Field

    Neuroscience

Mitochondrial Ferritin Antibody (YA3353) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using Mitochondrial Ferritin Antibody (HY-P83608) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Mitochondrial Ferritin Antibody (YA3353) Product Image
    1/16
  • Experimental Validation Results for Mitochondrial Ferritin Antibody (YA3353)
  • Mitochondrial Ferritin Antibody (YA3353) Product Image
  • Experimental Validation Results for Mitochondrial Ferritin Antibody (YA3353)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using Mitochondrial Ferritin Antibody (HY-P83608) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Mitochondrial Ferritin Antibody (YA3353)
  • Mitochondrial Ferritin Antibody (YA3353) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using Mitochondrial Ferritin Antibody (HY-P83608) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Mitochondrial Ferritin Antibody (YA3353) Product Image WB