PPCEL Antibody (YA3175)
(Synonyms: CMS22; Prolylendopeptidase-like)PPCEL Antibody (YA3175) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPCEL.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human, Mouse, Rat, Hamster
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
PPCEL Antibody (YA3175) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPCEL.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat, Hamster
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Observed Molecular WeightObserved band size: 72 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 84 kDa
Entrez Gene: 9581 Human ; 213760 Mouse ; 298771 Rat
SwissProt: Q4J6C6 Human ; Q8C167 Mouse ; Q5HZA6 Rat
OMIM: 606407 Human
Recombinant protein of human PPCEL/PREPL aa451-651.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PPCEL serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex. May play a role in the regulation of synaptic vesicle exocytosis[1][2][3][4][5].
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Subcellular Localization
Cytoplasm, cytosol; Golgi apparatus, trans-Golgi network; Cytoplasm, cytoskeleton; Golgi apparatus; Nucleus
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Expression
Tissue_specificity:Expressed in pyramidal neurons of the temporal cortex and neocortex (at protein level) (PubMed:23485813) . Widely expressed (PubMed:15913950, PubMed:16385448) . Expressed at higher level in brain, skeletal muscle, heart and kidney (PubMed:15913950, PubMed:16385448) . Expressed at the endplates in the neuromuscular junction (PubMed:24610330) -
Isoforms & Post-Translational Modification
Q4J6C6 has 4 isomers: Q4J6C6-1: 83927 Da (predicted); Q4J6C6-2: 76352 Da (predicted); Q4J6C6-3: 76757 Da (predicted); Q4J6C6-4: 73352 Da (predicted).
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Subunit
Homodimer (PubMed:16143824). Interacts with the AP-1 complex (PubMed:23321636)
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SwissProt ID
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Synonyms
CMS22; Prolylendopeptidase-like
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Research Field
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Documentation
[1]. Szeltner Z, et al. The PREPL A protein, a new member of the prolyl oligopeptidase family, lacking catalytic activity. Cell Mol Life Sci. 2005 Oct;62(19-20):2376-81. [Content Brief]
[2]. Jaeken J, et al. Deletion of PREPL, a gene encoding a putative serine oligopeptidase, in patients with hypotonia-cystinuria syndrome. Am J Hum Genet. 2006 Jan;78(1):38-51. [Content Brief]
[3]. Régal L, et al. PREPL deficiency: delineation of the phenotype and development of a functional blood assay. Genet Med. 2018 Jan;20(1):109-118. [Content Brief]
[4]. Radhakrishnan K, et al. Trans-Golgi network morphology and sorting is regulated by prolyl-oligopeptidase-like protein PREPL and the AP-1 complex subunit μ1A. J Cell Sci. 2013 Mar 1;126(Pt 5):1155-63. [Content Brief]
[5]. Régal L, et al. PREPL deficiency with or without cystinuria causes a novel myasthenic syndrome. Neurology. 2014 Apr 8;82(14):1254-60. [Content Brief]
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