TAB1 Antibody (YA5163)

Cat. No.: HY-P85471: Data Sheet User Guide for Antibodies
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TAB1 Antibody (YA5163) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TAB1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

TAB1 Antibody (YA5163) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TAB1.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 55 kDa;

Species Reactivity

Human, Mouse, Rat, Bovine, Dog, Pig

SwissProt ID

Q15750

Gene ID

10454 [NCBI]

Immunogen

Purified recombinant human TAB1 (N-terminus) protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Key adapter protein that plays an essential role in JNK and NF-kappa-B activation and proinflammatory cytokines production in response to stimulation with TLRs and cytokines (PubMed:22307082, PubMed:24403530). Mechanistically, associates with the catalytic domain of MAP3K7/TAK1 to trigger MAP3K7/TAK1 autophosphorylation leading to its full activation (PubMed:10838074, PubMed:25260751, PubMed:37832545). Similarly, associates with MAPK14 and triggers its autophosphorylation and subsequent activation (PubMed:11847341, PubMed:29229647). In turn, MAPK14 phosphorylates TAB1 and inhibits MAP3K7/TAK1 activation in a feedback control mechanism (PubMed:14592977). Also plays a role in recruiting MAPK14 to the TAK1 complex for the phosphorylation of the TAB2 and TAB3 regulatory subunits (PubMed:18021073)

Subcellular Localization：Cytoplasm, cytosol; Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：Q15750 has 2 isomers: Q15750-1: 54644 Da (predicted); Q15750-2: 49917 Da (predicted).
Phosphorylated at all three sites Ser-423, Thr-431 and Ser-438 by MAPK14 when cells were exposed to cellular stresses, or stimulated with TNF-alpha, IL1 or LPS (PubMed:14592977). These phosphorylations inhibit TAK1 activation by a feedback control mechanism (PubMed:14592977). Dephosphorylated by DUSP14 at Ser-438, leading to TAB1-MAP3K7/TAK1 complex inactivation in T-cells (PubMed:24403530);Ubiquitinated by MAP3K1 with 'Lys-63'-linked polyubiquitin; leading to activation of TAK1 and of JNK and p38 MAP kinases following EGF and TGF-beta stimulation (PubMed:25260751). Ubiquitinated by ITCH with 'Lys-48'-linked polyubiquitin; leading to proteasomal degradation (PubMed:25714464). Ubiquitinated by RNF114 during maternal-to-zygotic transition; leading to degradation (By similarity);(Microbial infection) Deubiquitinated by Y.enterocolitica YopP;O-GlcNAcylated at Ser-395 by OGT is required for full MAP3K7/TAK1 activation upon stimulation with IL-1 or osmotic stress (PubMed:22307082). Deglycosylated at Ser-395 by OGA (PubMed:28939839)

Subunit：Interacts with XIAP and BIRC7 (PubMed:11865055, PubMed:17560374). Interacts with TRAF6 and MAP3K7; during IL-1 signaling (PubMed:10094049, PubMed:10838074, PubMed:11323434, PubMed:8638164). Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2 (PubMed:11460167). Interacts with TRAF6 and MAPK14; these interactions allow MAPK14 autophosphorylation (PubMed:11847341). Interacts with STING1; interaction takes place following cGAMP activation and promotes TAB1 recruitment to the endoplasmic reticulum, triggering MAP3K7/TAK1 activation and STING1 phosphorylation (PubMed:37832545)

Synonyms

TAB1; MAP3K7IP1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TGF-beta-activated kinase 1-binding protein 1; TAK1-binding protein 1

각종 서류

User Guide for Antibodies (1077 KB)