Copy Link Print Information

Integrin alpha 2 Antibody (YA3107)

(Synonyms: ITGA2; CD49B; Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD49b)
Customer Review

Based on 1 Customer Validation

Integrin alpha 2 Antibody (YA3107) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Integrin alpha 2 Antibody (YA3107) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:100 1:50-1:100

Product Details

Description

Integrin alpha 2 Antibody (YA3107) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 129 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Integrin alpha 2 aa1100-1181.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105475

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse spleen (lane3, 20μg), Rat spleen (lane4, 20μg) and A431 (lane5, 20μg) using Integrin alpha 2 Antibody (HY-P83362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    Flow cytometric analysis of 1X10^6 A431 cells labeling Integrin alpha 2 Antibody (HY-P83362, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Integrin alpha 2 is an Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Subunit

    Heterodimer of an alpha and a beta subunit. Alpha-2 associates with beta-1. Interacts with HPS5 and RAB21

  • SwissProt ID

    P17301

  • Gene ID
    3673 [NCBI]
  • Synonyms

    ITGA2; CD49B; Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD49b

  • Research Field

    Signal Transduction

Integrin alpha 2 Antibody (YA3107) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse spleen (lane3, 20μg), Rat spleen (lane4, 20μg) and A431 (lane5, 20μg) using Integrin alpha 2 Antibody (HY-P83362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 A431 cells labeling Integrin alpha 2 Antibody (HY-P83362, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin alpha 2 Antibody (YA3107) Product Image
    1/16
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Integrin alpha 2 Antibody (YA3107) Product Image
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse spleen (lane3, 20μg), Rat spleen (lane4, 20μg) and A431 (lane5, 20μg) using Integrin alpha 2 Antibody (HY-P83362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 A431 cells labeling Integrin alpha 2 Antibody (HY-P83362, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Experimental Validation Results for Integrin alpha 2 Antibody (YA3107)
  • Integrin alpha 2 Antibody (YA3107) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse spleen (lane3, 20μg), Rat spleen (lane4, 20μg) and A431 (lane5, 20μg) using Integrin alpha 2 Antibody (HY-P83362). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Integrin alpha 2 Antibody (YA3107) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin alpha 2 Antibody (YA3107) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Integrin alpha 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Integrin alpha 2 Antibody (YA3107) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 A431 cells labeling Integrin alpha 2 Antibody (HY-P83362, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Integrin alpha 2 Antibody (YA3107) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin alpha 2 Antibody (YA3107) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Integrin alpha 2 with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin alpha 2 antibody (HY-P83362) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin alpha 2 Antibody (YA3107) Product Image WB
  • Integrin alpha 2 Antibody (YA3107) Product Image IHC-P
  • Integrin alpha 2 Antibody (YA3107) Product Image IHC-P
  • Integrin alpha 2 Antibody (YA3107) Product Image FC
  • Integrin alpha 2 Antibody (YA3107) Product Image ICC
  • Integrin alpha 2 Antibody (YA3107) Product Image ICC