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Clathrin Heavy Chain Antibody (YA501)

Customer Review

Based on 1 Customer Validation

Clathrin Heavy Chain Antibody (YA501) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Clathrin Heavy Chain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Clathrin Heavy Chain Antibody (YA501) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200

Product Details

Description

Clathrin Heavy Chain Antibody (YA501) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Clathrin Heavy Chain.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 180 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 180 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Clathrin heavy chain.AA range:201-250.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102297

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
    Western blot analysis of extracts from Hela (lane 2(20μg) ) , HUVEC (lane 3(20μg) and NIH3T3 (lane 4(20μg) ) using Clathrin heavy chain Rabbit mAb（HY-P80086）. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin , HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Clathrin Heavy Chain is a Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation. Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side; Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side; Melanosome; Cytoplasm, cytoskeleton, spindle

  • Subunit

    Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat (PubMed:16968737).

  • SwissProt ID

    Q00610

  • Gene ID
    1213 [NCBI]
  • Research Field

    Signal Transduction

Clathrin Heavy Chain Antibody (YA501) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) ) , HUVEC (lane 3(20μg) and NIH3T3 (lane 4(20μg) ) using Clathrin heavy chain Rabbit mAb（HY-P80086）. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin , HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Clathrin Heavy Chain Antibody (YA501) Product Image
    1/16
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Clathrin Heavy Chain Antibody (YA501) Product Image
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) ) , HUVEC (lane 3(20μg) and NIH3T3 (lane 4(20μg) ) using Clathrin heavy chain Rabbit mAb（HY-P80086）. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin , HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Experimental Validation Results for Clathrin Heavy Chain Antibody (YA501)
  • Clathrin Heavy Chain Antibody (YA501) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) ) , HUVEC (lane 3(20μg) and NIH3T3 (lane 4(20μg) ) using Clathrin heavy chain Rabbit mAb（HY-P80086）. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin , HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Clathrin Heavy Chain Antibody (YA501) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Clathrin Heavy Chain Antibody (YA501) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Clathrin heavy chain Antibody. The section was pre-treated using heat mediated antigen retrieval with Trsi-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80086, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Clathrin Heavy Chain Antibody (YA501) Product Image WB
  • Clathrin Heavy Chain Antibody (YA501) Product Image IHC-P
  • Clathrin Heavy Chain Antibody (YA501) Product Image IHC-P