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StAR Antibody (YA3542)

(Synonyms: StARD1; Cholesterol trafficker; Luteinizing hormone induced protein; Mitochondrial steroid acute regulatory protein; StAR related lipid transfer (START) domain containing 1; StARD1; START domain containing protein 1; Steroidogenic Acute Regulatory Protein; Steroidogenic acute regulatory protein mitochondrial; STAR_HUMAN.)
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Based on 1 Customer Validation

StAR Antibody (YA3542) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to StAR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for StAR Antibody (YA3542)
  • StAR Antibody (YA3542) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

StAR Antibody (YA3542) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to StAR.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human StAR.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103265

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for StAR Antibody (YA3542)
    Western blot analysis of extracts from K562(lane 2, 20μg), THP-1(lane 3, 20μg), Mouse Liver(lane 4, 20μg) and MOLT-4 (lane 5, 20μg) using StAR Antibody (HY-P81192). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

Background

  • Function

    StAR plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone

  • Subcellular Localization

    Mitochondrion

  • Expression


    Tissue_specificity:Expressed in gonads, adrenal cortex and kidney

  • Subunit

    May interact with TSPO

  • SwissProt ID

    P49675

  • Gene ID
    6770 [NCBI]
  • Synonyms

    StARD1; Cholesterol trafficker; Luteinizing hormone induced protein; Mitochondrial steroid acute regulatory protein; StAR related lipid transfer (START) domain containing 1; StARD1; START domain containing protein 1; Steroidogenic Acute Regulatory Protein; Steroidogenic acute regulatory protein mitochondrial; STAR_HUMAN.

StAR Antibody (YA3542) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2, 20μg), THP-1(lane 3, 20μg), Mouse Liver(lane 4, 20μg) and MOLT-4 (lane 5, 20μg) using StAR Antibody (HY-P81192). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • StAR Antibody (YA3542) Product Image
    1/16
  • Experimental Validation Results for StAR Antibody (YA3542)
  • StAR Antibody (YA3542) Product Image
  • Experimental Validation Results for StAR Antibody (YA3542)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2, 20μg), THP-1(lane 3, 20μg), Mouse Liver(lane 4, 20μg) and MOLT-4 (lane 5, 20μg) using StAR Antibody (HY-P81192). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for StAR Antibody (YA3542)
  • StAR Antibody (YA3542) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2, 20μg), THP-1(lane 3, 20μg), Mouse Liver(lane 4, 20μg) and MOLT-4 (lane 5, 20μg) using StAR Antibody (HY-P81192). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • StAR Antibody (YA3542) Product Image WB