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GPX4 Antibody (YA9649)(PBS only)

(Synonyms: Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; PHGPx; EC 1.11.1.12; Glutathione peroxidase 4; GPx-4; GSHPx-4)
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GPX4 Antibody(YA9649)(PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPX4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • GPX4 Antibody (YA9649)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:100-1:20 1:50-1:200

Product Details

Description

GPX4 Antibody(YA9649)(PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPX4.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human GPX4 aa18-65.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    GPX4 (glutathione peroxidase 4) is a selenium-dependent phospholipid hydroperoxidase that maintains cellular redox homeostasis by reducing complex lipid hydroperoxides to their corresponding lipid alcohols using glutathione as a cofactor, thereby preventing membrane lipid peroxidation and oxidative damage[1][2][3]. Mechanistically, GPX4 functions as the central regulator and major endogenous suppressor of ferroptosis, an iron-dependent form of regulated cell death driven by the accumulation of phospholipid hydroperoxides, and loss of GPX4 activity promotes lipid peroxide accumulation and ferroptotic cell death[2][4][5][6]. Through this lipid peroxide detoxification pathway, GPX4 contributes to the maintenance of cellular viability, immune homeostasis, and tissue integrity, while impaired GPX4 function has been associated with cancer, neurodegenerative disorders, inflammation, ischemia-reperfusion injury, and other ferroptosis-related pathologies[1][7][8]. Compared with other glutathione peroxidase family members, GPX4 possesses a unique ability to directly reduce phospholipid hydroperoxides and oxidized complex lipids, distinguishing it as a specialized phospholipid repair enzyme and a master regulator of ferroptosis[2][9][10]. Distinct cytosolic, mitochondrial, and nuclear GPX4 isoforms have been described, with cytosolic GPX4 identified as essential for embryonic development and cell survival[7][8]. For experimental applications, pharmacological GPX4 inhibitors such as RSL3 and ML162 are widely used to induce ferroptosis, enabling mechanistic studies of lipid peroxidation, redox regulation, cancer vulnerability, and ferroptosis-targeted therapeutic strategies[11][12].

  • Subcellular Localization

    Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:Present primarily in testis. Expressed in platelets (at protein level).

  • Isoforms & Post-Translational Modification

    P36969 has 2 isomers: P36969-1: 22175 Da (predicted); P36969-2: 19525 Da (predicted).
    Degraded via chaperone-mediated autophagy, promoting ferroptosis. Hydroxylation by EGLN3/PHD3 promotes its stabilization by preventing degradation via chaperone-mediated autophagy.

  • Subunit

    Monomer. Has a tendency to form higher mass oligomers (PubMed:17630701). Interacts with FUNDC1; this interaction promotes GPX4 recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy (PubMed:36828120). Interacts (via KFERQ motif) with HSPA8; promoting its association with LAMP2 and degradation via chaperone-mediated autophagy (By similarity).

  • SwissProt ID

    P36969

  • Gene ID
    2879 [NCBI]
  • Synonyms

    Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; PHGPx; EC 1.11.1.12; Glutathione peroxidase 4; GPx-4; GSHPx-4

  • Research Field

    Signal Transduction

References

[1]. Weaver K, et al. The Selenoprotein Glutathione Peroxidase 4: From Molecular Mechanisms to Novel Therapeutic Opportunities. Biomedicines. 2022 Apr 13;10(4):891. [Content Brief]

[2]. Ma T, et al. GPX4-independent ferroptosis-a new strategy in disease's therapy. Cell Death Discov. 2022 Oct 30;8(1):434. [Content Brief]

[3]. Xia Y, et al. SIRT1 activation ameliorates rhesus monkey liver fibrosis by inhibiting the TGF-β/smad signaling pathway. Chem Biol Interact. 2024 May 1;394:110979. [Content Brief]

[4]. Li J, et al. Ferroptosis: past, present and future. Cell Death Dis. 2020 Feb 3;11(2):88. [Content Brief]

[5]. Ursini F, et al. Lipid peroxidation and ferroptosis: The role of GSH and GPx4. Free Radic Biol Med. 2020 May 20;152:175-185. [Content Brief]

[6]. Ursini F, et al. A white paper on Phospholipid Hydroperoxide Glutathione Peroxidase (GPx4) forty years later. Free Radic Biol Med. 2022 Aug 1;188:117-133. [Content Brief]

[7]. Xie Y, et al. GPX4 in cell death, autophagy, and disease. Autophagy. 2023 Oct;19(10):2621-2638. [Content Brief]

[8]. GPX4 gene information from NCBI.

[9]. Liu Y, et al. GPX4: The hub of lipid oxidation, ferroptosis, disease and treatment. Biochim Biophys Acta Rev Cancer. 2023 May;1878(3):188890. [Content Brief]

[10]. Sciencedirect.topics.

[11]. Cui C, et al. Post-Translational Modification of GPX4 is a Promising Target for Treating Ferroptosis-Related Diseases. Front Mol Biosci. 2022 May 12;9:901565. [Content Brief]

[12]. Sekhar KR, et al. Glutathione peroxidase 4 inhibition induces ferroptosis and mTOR pathway suppression in thyroid cancer. Sci Rep. 2022 Nov 12;12(1):19396. [Content Brief]

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GPX4 Antibody (YA9649)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • GPX4 Antibody (YA9649)(PBS only) Product Image