c-fos Antibody (YA9752)
(Synonyms: Activator protein 1, AP 1, C FOS, Cellular oncogene c fos, Cellular oncogene fos, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FBJ murine osteosarcoma viral v fos oncogene homolog, FBJ Osteosarcoma Virus, FOS)
Based on 1 Customer Validation
c-fos Antibody (YA9752) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to c-fos.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF, IP, ChIP, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ChIP
ChIP: Chromatin Immunoprecipitation
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|---|---|
| Dilution Ratio | 1:2000-5000 | 1:4000-10000 | 1:1000-2000 | 1:4000-10000 | 1:5000 | Use 0.5~2 μg for 25 μg of chromatin | 1-2μg/sample |
Product Details
c-fos Antibody (YA9752) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to c-fos.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat Predicted Reactivity: Monkey,Pig
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 41-55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 41 kDa
Recombinant protein of human Protein c-Fos (aa 1-380).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
c-fos is a Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum
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Subcellular Localization
Nucleus; Endoplasmic reticulum; Cytoplasm, cytosol
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Isoforms & Post-Translational Modification
c-fos has 3 isoforms, P01100-1: amino acid length is 380, molecular weight is 40695 Da (predicted); P01100-2: amino acid length is 266, molecular weight is 28986 Da (predicted); P01100-3: amino acid length is 344, molecular weight is 36301 Da (predicted).
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. -
Subunit
Heterodimer; with JUN (By similarity).
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SwissProt ID
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Synonyms
Activator protein 1, AP 1, C FOS, Cellular oncogene c fos, Cellular oncogene fos, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FBJ murine osteosarcoma viral v fos oncogene homolog, FBJ Osteosarcoma Virus, FOS
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