Acetyl-Histone H2B (Lys20) Antibody (YA9936)
(Synonyms: GL105; H2B GL105; H2B histone family member O; H2B histone family member S; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e)
Based on 1 Customer Validation
Acetyl-Histone H2B (Lys20) Antibody (YA9936) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Acetyl-Histone H2B (Lys20).
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, ICC/IF, ChIP
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
ChIP
ChIP: Chromatin Immunoprecipitation
|
|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:50 | 1:1000 | Recomend 0.5~2 μg for 25 μg of chromatin |
Product Details
Acetyl-Histone H2B (Lys20) Antibody (YA9936) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Acetyl-Histone H2B (Lys20).
-
Host Rabbit
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 14 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 14 kDa
Synthetic Acetylpeptide corresponding to residues surrounding Lys20 of human Histone H2B protein.
Endogenous
affinity purified
Non-conjugated
Acetylated
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Acetyl-Histone H2B is a Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
-
Subcellular Localization
Nucleus; Chromosome
-
Isoforms & Post-Translational Modification
Acetyl-Histone H2B has an amino acid length of 126, molecular weight is 13890 Da.
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. -
Subunit
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
-
SwissProt ID
-
Synonyms
GL105; H2B GL105; H2B histone family member O; H2B histone family member S; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e
Documentation
Powered by Bioz
