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ENT2 Antibody (YA2442)

(Synonyms: DER12; ei-type; HNP36; Nucleoside transporter; SLC29A2)
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Based on 1 Customer Validation

ENT2 Antibody (YA2442) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENT2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ENT2 Antibody (YA2442)
  • ENT2 Antibody (YA2442) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100 1:1000

Product Details

Description

ENT2 Antibody (YA2442) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENT2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55-65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within human ENT2 aa 1-12/456.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ENT2 Antibody (YA2442)
    Western blot analysis of extracts from Hela (lane2, 20μg), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using ENT2 Antibody (HY-P82697). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Equilibrative nucleoside transporter 2 (ENT2, SLC29A2) is a broadly expressed equilibrative transporter that mediates bidirectional flux of purine and pyrimidine nucleosides as well as multiple nucleobases, thereby supporting nucleoside homeostasis and salvage pathways of nucleotide synthesis[1][2]. ENT2 functions as a sodium-independent membrane transporter and contributes to the regulation of extracellular and intracellular pools of adenosine, inosine, hypoxanthine, and related metabolites that influence cellular metabolism and signaling[1]. Mechanistically, ENT2 participates in nucleoside transport processes that affect adenosine receptor-dependent signaling and tissue responses to metabolic stress[1][3]. In disease-relevant settings, ENT2 has been implicated in intestinal epithelial adenosine regulation, where repression or deletion of ENT2 enhances mucosal A2B receptor signaling associated with resolution of intestinal inflammation[3]. ENT2 also contributes to the cellular disposition of therapeutic nucleoside analogs, including anticancer and antiviral agents, making transporter activity an important determinant of drug uptake and pharmacological response[1][2]. Compared with the closely related isoform ENT1, ENT2 shares broad nucleoside selectivity but displays efficient transport of nucleobases and is characteristically insensitive to nitrobenzylmercaptopurine riboside (NBMPR), a distinction widely used for functional discrimination in transport studies[1][2]. For experimental applications, NBMPR sensitivity profiles, together with dipyridamole and dilazep inhibition studies, are commonly used to distinguish ENT family transporter activity and to investigate nucleoside transport mechanisms in cellular models[1].

  • Subcellular Localization

    Apical cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Highly expressed in skeletal muscle (PubMed:9478986) . Expressed in liver, lung, placenta, brain, heart, kidney and ovarian tissues (PubMed:9478986) . Expressed in testis at the blood-brain-barrier (PubMed:23639800)
    Positive sample: HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, C2C12 cell lysate, L6 cell lysate, Mouse lung tissue lysate, HepG2, C2C12.

  • Isoforms & Post-Translational Modification

    Q14542 has 4 isomers: Q14542-1: 50113 Da (predicted); Q14542-2: 22276 Da (predicted); Q14542-3: 32507 Da (predicted); Q14542-4: 38758 Da (predicted).
    Glycosylated

  • SwissProt ID

    Q14542

  • Gene ID
    3177 [NCBI]
  • Synonyms

    DER12; ei-type; HNP36; Nucleoside transporter; SLC29A2

  • Research Field

    Signal Transduction

References

[1]. Naes SM, et al. Equilibrative Nucleoside Transporter 2: Properties and Physiological Roles. Biomed Res Int. 2020 Dec 3;2020:5197626. [Content Brief]

[2]. Uniprotkb.

[3]. Gatchalian J, et al. Racheting Up Repair. Immunity. 2018 Oct 16;49(4):577-579. [Content Brief]

ENT2 Antibody (YA2442) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using ENT2 Antibody (HY-P82697). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • ENT2 Antibody (YA2442) Product Image
    1/16
  • Experimental Validation Results for ENT2 Antibody (YA2442)
  • ENT2 Antibody (YA2442) Product Image
  • Experimental Validation Results for ENT2 Antibody (YA2442)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using ENT2 Antibody (HY-P82697). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENT2 Antibody (YA2442)
  • ENT2 Antibody (YA2442) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using ENT2 Antibody (HY-P82697). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • ENT2 Antibody (YA2442) Product Image WB