ALKBH2 Antibody (YA2047)(PBS only)
(Synonyms: ABH2)ALKBH2 Antibody (YA2047) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALKBH2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
ALKBH2 Antibody (YA2047) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALKBH2.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 29 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 29 kDa
A synthetic peptide of human ALKBH2 aa15-29.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ALKBH2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
ALKBH2 is a Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription. In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus; Nucleus, nucleolus; Nucleus, nucleoplasm
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Expression
Tissue_specificity:Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder -
Isoforms & Post-Translational Modification
Q6NS38 has 2 isomers: Q6NS38-1: 29322 Da (predicted); Q6NS38-2: 17083 Da (predicted).
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Subunit
Interacts with PCNA homotrimer; this interaction is enhanced during the S-phase of the cell cycle (PubMed:19736315, PubMed:23972994, PubMed:26408825). Interacts with nucleolar proteins NCL, UBTF and NPM1 (PubMed:23972994). Interacts with XRCC5-XRCC6 heterodimer (PubMed:23972994)
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SwissProt ID
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Synonyms
ABH2
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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