Copy Link Print Information

Prostatic Acid Phosphatase Antibody (YA9887)

(Synonyms: 5' -NT; 5' -nucleotidase; ACP3; PAP; Prostatic acid phosphatase; TMPase)
Customer Review

Based on 1 Customer Validation

Prostatic Acid Phosphatase Antibody (YA9887) is a Mouse-derived and non-conjugated 0 Monoclonal antibody, targeting to Prostatic Acid Phosphatase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4),0.1% BSA, 40% Glycerol, 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Prostatic Acid Phosphatase Antibody (YA9887)
  • Prostatic Acid Phosphatase Antibody (YA9887) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:5000 1:5000

Product Details

Description

Prostatic Acid Phosphatase Antibody (YA9887) is a Mouse-derived and non-conjugated 0 Monoclonal antibody, targeting to Prostatic Acid Phosphatase.

  • Host Mouse
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 45 kDa
Immunogen

Synthesized peptide derived from human PSAP AA range: 201-300.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4),0.1% BSA, 40% Glycerol, 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Prostatic Acid Phosphatase Antibody (YA9887)
    Western blot analysis of extracts from U-251MG (lane 2, 20ug), MCF-7 (lane 3, 20ug), PC-3 (lane 4, 20ug) and A549 (lane 5, 20ug) using Prostatic Acid Phosphatase Antibody (HY-P810625). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Prostatic Acid Phosphatase is an A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma[1][2][3][4][5].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_Specificity: Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues.

  • Isoforms & Post-Translational Modification

    Prostatic Acid Phosphatase has 3 isoforms, P15309-1: amino acid length is 386, molecular weight is 44566 Da (predicted); P15309-2: amino acid length is 418, molecular weight is 48336 Da (predicted); P15309-3: amino acid length is 353, molecular weight is 40443 Da (predicted).
    N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.

  • Subunit

    Homodimer; dimer formation is required for phosphatase activity

  • SwissProt ID

    P15309

  • Gene ID
    55 [NCBI]
  • Synonyms

    5' -NT; 5' -nucleotidase; ACP3; PAP; Prostatic acid phosphatase; TMPase

References

[1]. Hiroyama M, et al. Isolation of a cDNA encoding human lysophosphatidic acid phosphatase that is involved in the regulation of mitochondrial lipid biosynthesis. J Biol Chem. 1999 Oct 8;274(41):29172-80. [Content Brief]

[2]. Tanaka M, et al. Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma. FEBS Lett. 2004 Jul 30;571(1-3):197-204. [Content Brief]

[3]. Chuang TD, et al. Human prostatic acid phosphatase, an authentic tyrosine phosphatase, dephosphorylates ErbB-2 and regulates prostate cancer cell growth. J Biol Chem. 2010 Jul 30;285(31):23598-606. [Content Brief]

[4]. Zhang Z, et al. Covalent modification and site-directed mutagenesis of an active site tryptophan of human prostatic acid phosphatase. Acta Biochim Pol. 1997;44(4):659-72. [Content Brief]

[5]. Ostanin K, et al. Heterologous expression of human prostatic acid phosphatase and site-directed mutagenesis of the enzyme active site. J Biol Chem. 1994 Mar 25;269(12):8971-8. [Content Brief]

View More

Prostatic Acid Phosphatase Antibody (YA9887) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U-251MG (lane 2, 20ug), MCF-7 (lane 3, 20ug), PC-3 (lane 4, 20ug) and A549 (lane 5, 20ug) using Prostatic Acid Phosphatase Antibody (HY-P810625). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Prostatic Acid Phosphatase Antibody (YA9887) Product Image
    1/16
  • Experimental Validation Results for Prostatic Acid Phosphatase Antibody (YA9887)
  • Prostatic Acid Phosphatase Antibody (YA9887) Product Image
  • Experimental Validation Results for Prostatic Acid Phosphatase Antibody (YA9887)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U-251MG (lane 2, 20ug), MCF-7 (lane 3, 20ug), PC-3 (lane 4, 20ug) and A549 (lane 5, 20ug) using Prostatic Acid Phosphatase Antibody (HY-P810625). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Prostatic Acid Phosphatase Antibody (YA9887)
  • Prostatic Acid Phosphatase Antibody (YA9887) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U-251MG (lane 2, 20ug), MCF-7 (lane 3, 20ug), PC-3 (lane 4, 20ug) and A549 (lane 5, 20ug) using Prostatic Acid Phosphatase Antibody (HY-P810625). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Prostatic Acid Phosphatase Antibody (YA9887) Product Image WB