PRKRA Antibody (YA1522)
(Synonyms: RAX; PACT; DYT16; HSD14)PRKRA Antibody (YA1522) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRKRA.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
PRKRA Antibody (YA1522) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRKRA.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 34 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 34 kDa
Entrez Gene: 8575 Human ; 23992 Mouse ; 311130 Rat
SwissProt: O75569 Human ; Q9WTX2 Mouse ; Q4V8C7 Rat
OMIM: 612067 Human
A synthetic peptide of of human PACT (phospho KR activating protein)/PRKRA
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner
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Subcellular Localization
Cytoplasm, perinuclear region; Cytoplasm
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Isoforms & Post-Translational Modification
O75569 has 3 isomers: O75569-1: 34404 Da (predicted); O75569-2: 33123 Da (predicted); O75569-3: 31635 Da (predicted).
Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress -
Subunit
Homodimer. Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DICER1, AGO2 and TARBP2. Also able to interact with dsRNA. Interacts with UBC9 (By similarity). Forms a complex with UBC9 and p53/TP53 (By similarity). Interacts with DUS2L (via DRBM domain). Interacts with RIGI
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SwissProt ID
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Synonyms
RAX; PACT; DYT16; HSD14
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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