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Fibrinogen gamma Chain Antibody (YA1896)

(Synonyms: FGG; Fibrinogen gamma chain)
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Based on 1 Customer Validation

Fibrinogen gamma Chain Antibody (YA1896) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibrinogen gamma Chain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Fibrinogen gamma Chain Antibody (YA1896)
  • Fibrinogen gamma Chain Antibody (YA1896) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Fibrinogen gamma Chain Antibody (YA1896) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibrinogen gamma Chain.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Species Reactivity Database

Entrez Gene: 2266 Human

SwissProt: P02679 Human

Immunogen

A synthesized peptide derived from human Fibrinogen gamma chain aa400-453/453.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104264

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Fibrinogen gamma Chain Antibody (YA1896)
    Western blot analysis of extracts from HepG2(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) , HCT116(lane 5(20ug) using Fibrinogen gamma Chain Antibody (HY-P82151) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Fibrinogen gamma Chain is a Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Protein levels were detected in the plasma.

  • Isoforms & Post-Translational Modification

    P02679 has 2 isomers: P02679-1: 51512 Da (predicted); P02679-2: 49497 Da (predicted).
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers;Sulfation of C-terminal tyrosines increases affinity for thrombin

  • Subunit

    Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain

  • SwissProt ID

    P02679

  • Gene ID
    2266 [NCBI]
  • Synonyms

    FGG; Fibrinogen gamma chain

  • Research Field

    Cardiovascular

Fibrinogen gamma Chain Antibody (YA1896) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) , HCT116(lane 5(20ug) using Fibrinogen gamma Chain Antibody (HY-P82151) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Fibrinogen gamma Chain Antibody (YA1896) Product Image
    1/16
  • Experimental Validation Results for Fibrinogen gamma Chain Antibody (YA1896)
  • Fibrinogen gamma Chain Antibody (YA1896) Product Image
  • Experimental Validation Results for Fibrinogen gamma Chain Antibody (YA1896)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) , HCT116(lane 5(20ug) using Fibrinogen gamma Chain Antibody (HY-P82151) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Fibrinogen gamma Chain Antibody (YA1896)
  • Fibrinogen gamma Chain Antibody (YA1896) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) , HCT116(lane 5(20ug) using Fibrinogen gamma Chain Antibody (HY-P82151) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Fibrinogen gamma Chain Antibody (YA1896) Product Image WB