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COL5A1 Antibody (YA6511)

(Synonyms: Collagen alpha-1(V) chain COL5A1)
Customer Review

Based on 1 Customer Validation

COL5A1 Antibody (YA6511) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COL5A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • COL5A1 Antibody (YA6511) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:100 1:1000 1-2μg/sample

Product Details

Description

COL5A1 Antibody (YA6511) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COL5A1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 250 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 184 kDa
Immunogen

Recombinant protein within human COL5A1 aa 251-450.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719217

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for COL5A1 Antibody (YA6511)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
    Western blot analysis of extracts from HeLa (lane 1(30μg)) 、HT1080 (lane 2(30μg)) 、HL-60 (lane 3(30μg)) and THP-1 (lane 4(30μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
    Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4), and HT-1080 (lane 5) using COL5A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    COL5A1 is a Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Isoforms & Post-Translational Modification

    P20908 has two isomers: P20908-1: 183560 Da (predicted); P20908-2: 183653 Da (predicted).
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains丨Sulfated on 40% of tyrosines

  • Subunit

    Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta

  • SwissProt ID

    P20908

  • Gene ID
    1289 [NCBI]
  • Synonyms

    Collagen alpha-1(V) chain COL5A1

COL5A1 Antibody (YA6511) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(30μg)) 、HT1080 (lane 2(30μg)) 、HL-60 (lane 3(30μg)) and THP-1 (lane 4(30μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4), and HT-1080 (lane 5) using COL5A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • COL5A1 Antibody (YA6511) Product Image
    1/16
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • COL5A1 Antibody (YA6511) Product Image
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(30μg)) 、HT1080 (lane 2(30μg)) 、HL-60 (lane 3(30μg)) and THP-1 (lane 4(30μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4), and HT-1080 (lane 5) using COL5A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • Experimental Validation Results for COL5A1 Antibody (YA6511)
  • COL5A1 Antibody (YA6511) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • COL5A1 Antibody (YA6511) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(30μg)) 、HT1080 (lane 2(30μg)) 、HL-60 (lane 3(30μg)) and THP-1 (lane 4(30μg)) using COL5A1 Antibody (HY-P86818) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • COL5A1 Antibody (YA6511) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (35 μg) from HeLa (lane 2), SH-SY5Y (lane 3), 293T (lane 4), and HT-1080 (lane 5) using COL5A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • COL5A1 Antibody (YA6511) Product Image WB
  • COL5A1 Antibody (YA6511) Product Image WB
  • COL5A1 Antibody (YA6511) Product Image WB