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PI 3 Kinase p150 Antibody (YA2823)

(Synonyms: P150; Phosphoinositide 3 kinase regulatory subunit 4; PI3 kinase p150 subunit; PIK3R4; VPS15)
Customer Review

Based on 1 Customer Validation

PI 3 Kinase p150 Antibody (YA2823) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PI 3 Kinase p150.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • PI 3 Kinase p150 Antibody (YA2823) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

PI 3 Kinase p150 Antibody (YA2823) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PI 3 Kinase p150.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 153 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PI 3 Kinase R4 aa1300-1358.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105194

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
    Western blot analysis of extracts from Jurkat (lane 2(20μg) and Jurkat (lane 3(40μg) using PI 3 Kinase p150 (HY-P83078) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PI 3 Kinase p150 is a Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2[1].

  • Subcellular Localization

    Late endosome; Cytoplasmic vesicle, autophagosome; Membrane; Lipid-anchor

  • Expression


    Tissue_specificity:general expression

  • Subunit

    Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxiliary subunits to form alternative complex forms. Alternative complex forms containing a fourth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG (PubMed:19270696, PubMed:23878393, PubMed:25490155, PubMed:8999962). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2 (PubMed:19270696, PubMed:20643123, PubMed:24785657). PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3C3/VPS34 (PubMed:14617358). Interacts with NRBF2 (PubMed:24785657). Interacts with ARMC3 (By similarity)

  • SwissProt ID

    Q99570

  • Gene ID
    30849 [NCBI]
  • Synonyms

    P150; Phosphoinositide 3 kinase regulatory subunit 4; PI3 kinase p150 subunit; PIK3R4; VPS15

  • Research Field

    Signal Transduction

PI 3 Kinase p150 Antibody (YA2823) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg) and Jurkat (lane 3(40μg) using PI 3 Kinase p150 (HY-P83078) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PI 3 Kinase p150 Antibody (YA2823) Product Image
    1/16
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • PI 3 Kinase p150 Antibody (YA2823) Product Image
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg) and Jurkat (lane 3(40μg) using PI 3 Kinase p150 (HY-P83078) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • Experimental Validation Results for PI 3 Kinase p150 Antibody (YA2823)
  • PI 3 Kinase p150 Antibody (YA2823) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg) and Jurkat (lane 3(40μg) using PI 3 Kinase p150 (HY-P83078) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PI 3 Kinase p150 Antibody (YA2823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PI 3 Kinase p150 Antibody (YA2823) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using PI 3 Kinase p150 Antibody (YA2823). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83078, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PI 3 Kinase p150 Antibody (YA2823) Product Image WB
  • PI 3 Kinase p150 Antibody (YA2823) Product Image IHC-P
  • PI 3 Kinase p150 Antibody (YA2823) Product Image IHC-P