Cortactin Antibody (YA4797)
(Synonyms: CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1)Cortactin Antibody (YA4797) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cortactin.
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Host:
Mouse
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IF
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:200-400 | ICC/IF: 1:200-400 | 1:5000-20000 |
Product Details
Cortactin Antibody (YA4797) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cortactin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Calculated Molecular Weight Predicted band size: 62 kDa;
Purified recombinant fragment of human Cortactin expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Cortactin contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane. Plays a role in the invasiveness of cancer cells, and the formation of metastases[1][2][3][4][5].
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Subcellular Localization
Cytoplasm, cytoskeleton; Cell projection, lamellipodium; Cell projection, ruffle; Cell projection, dendrite; Cell projection; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, podosome; Cell junction; Cell junction, focal adhesion; Membrane, clathrin-coated pit; Cell projection, dendritic spine; Cytoplasm, cell cortex; Endoplasmic reticulum
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Isoforms & Post-Translational Modification
Q14247 has 3 isomers: Q14247-1: 61586 Da (predicted); Q14247-2: 70959 Da (predicted); Q14247-3: 57467 Da (predicted).
Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associates with filamentous actin (By similarity). Phosphorylated on tyrosine residues in response to CHRM1 activation (By similarity). Phosphorylated by PTK2/FAK1 in response to cell adhesion (By similarity). Phosphorylated by FER. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding;Acetylated -
Subunit
Part of a complex composed of NEDD9, AURKA and CTTN; within the complex NEDD9 acts as a scaffold protein and is required for complex formation (PubMed:24574519). Interacts (via N-terminus) with NEDD9 (PubMed:24574519). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Forms a complex made of ABL1 and MYLK (PubMed:20861316). Interacts with SHANK2 and SHANK3 (via its SH3 domain). Interacts with FGD1. Interacts with ABL2 (By similarity). Interacts with KCNA2 (via non-phosphorylated C-terminus) (PubMed:12151401). Interacts with PLXDC2 and SRCIN1. Interacts with SAMSN1 (via SH3 domain). Interacts (via SH3 domain) with ASAP1 (via Pro-rich region). Interacts (via SH3 domain) with DNM2 (By similarity). Interacts with ACTN1 (By similarity). Interacts with FER (PubMed:9722593). Interacts with CTTNBP2NL; this interaction may target CTTN to stress fibers. Interacts with CTTNBP2; this interaction may target CTTN at the cell cortex or dendritic spines. Interacts with KCNH1 (PubMed:23144454). Interacts (via SH3 domain) with DIP2A (via N-terminus); the interaction enhances CTTN acetylation and is required for proper synaptic transmission (By similarity). Interacts with XIRP1 (via N-terminus); the interaction promotes CTTN localization to intercalated disks in cardiomyocytes (By similarity)
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SwissProt ID
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Synonyms
CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1
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