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MRP1 Antibody (YA1583)

(Synonyms: ABC29; Abcc1; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP1)
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Based on 1 Customer Validation

MRP1 Antibody (YA1583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MRP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MRP1 Antibody (YA1583)
  • Experimental Validation Results for MRP1 Antibody (YA1583)
  • MRP1 Antibody (YA1583) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

MRP1 Antibody (YA1583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MRP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 250 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 172 kDa
Species Reactivity Database

Entrez Gene: 4363 Human

SwissProt: P33527 Human

Immunogen

A synthesized peptide derived from human MRP1 aa1500-1531.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103938

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MRP1 Antibody (YA1583)
    Western blot analysis was performed on extracts from A549 (lane 1, 20 μg), K562 (lane 2, 20 μg), HepG2 (lane 3, 20 μg), A431 (lane 4, 20 μg), 3T3 (lane 5, 20 μg), and Hela (lane 6, 20 μg) using MRP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MRP1 Antibody (YA1583)
    Flow cytometric analysis of 1X106 A549 cells labeling MRP1 Antibody(HY-P81838, red). Cells stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MRP1 mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthesizing cells. Mediates ATP-dependent, GSH-independent cyclic GMP-AMP (cGAMP) export. Thus, by limiting intracellular cGAMP concentrations negatively regulates the cGAS-STING pathway. Exports S-geranylgeranyl-glutathione (GGG) in lymphoid cells and stromal compartments of lymphoid organs. ABCC1 (via extracellular transport) with GGT5 (via GGG catabolism) establish GGG gradients within lymphoid tissues to position P2RY8-positive lymphocytes at germinal centers in lymphoid follicles and restrict their chemotactic transmigration from blood vessels to the bone marrow parenchyma. Mediates basolateral export of GSH-conjugated R- and S-prostaglandin A2 diastereomers in polarized epithelial cells[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Lung, testis and peripheral blood mononuclear cells

  • Isoforms & Post-Translational Modification

    P33527 has 9 isomers: P33527-1: 171591 Da (predicted); P33527-2: 164971 Da (predicted); P33527-3: 165569 Da (predicted); P33527-4: 164304 Da (predicted); P33527-5: 158949 Da (predicted); P33527-6: 157683 Da (predicted); P33527-7: 158282 Da (predicted); P33527-8: 151661 Da (predicted); P33527-9: 172640 Da (predicted).

  • Subunit

    (Microbial infection) Interacts with human cytomegalovirus protein UL138; this interaction mediates MRP1 degradation via the lysosome

  • SwissProt ID

    P33527

  • Gene ID
    4363 [NCBI]
  • Synonyms

    ABC29; Abcc1; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP1

  • Research Field

    Signal Transduction

MRP1 Antibody (YA1583) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 20 μg), K562 (lane 2, 20 μg), HepG2 (lane 3, 20 μg), A431 (lane 4, 20 μg), 3T3 (lane 5, 20 μg), and Hela (lane 6, 20 μg) using MRP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 A549 cells labeling MRP1 Antibody(HY-P81838, red). Cells stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MRP1 Antibody (YA1583) Product Image
    1/16
  • Experimental Validation Results for MRP1 Antibody (YA1583)
  • Experimental Validation Results for MRP1 Antibody (YA1583)
  • MRP1 Antibody (YA1583) Product Image
  • Experimental Validation Results for MRP1 Antibody (YA1583)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 20 μg), K562 (lane 2, 20 μg), HepG2 (lane 3, 20 μg), A431 (lane 4, 20 μg), 3T3 (lane 5, 20 μg), and Hela (lane 6, 20 μg) using MRP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MRP1 Antibody (YA1583)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 A549 cells labeling MRP1 Antibody(HY-P81838, red). Cells stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MRP1 Antibody (YA1583)
  • Experimental Validation Results for MRP1 Antibody (YA1583)
  • MRP1 Antibody (YA1583) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 20 μg), K562 (lane 2, 20 μg), HepG2 (lane 3, 20 μg), A431 (lane 4, 20 μg), 3T3 (lane 5, 20 μg), and Hela (lane 6, 20 μg) using MRP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MRP1 Antibody (YA1583) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 A549 cells labeling MRP1 Antibody(HY-P81838, red). Cells stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MRP1 Antibody (YA1583) Product Image WB
  • MRP1 Antibody (YA1583) Product Image FC