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PYCR2 Antibody (YA7952)

(Synonyms: HLD10; P5CR2)
Customer Review

Based on 1 Customer Validation

PYCR2 Antibody (YA7952) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PYCR2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • PYCR2 Antibody (YA7952) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:150-500 1:100-250 1:100

Product Details

Description

PYCR2 Antibody (YA7952) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to PYCR2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 33.5 kDa
Immunogen

Full length human recombinant protein of human PYCR2 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PYCR2 Antibody (YA7952)
    Western blot analysis of extracts from HEK293T(lane 2(20μg) , HepG2(lane 3(20μg) ,K562(lane 4(20μg)and Jurkat( lane 5(20μg) using PYCR2 Antibody (HY-P88268). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
    Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
    Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PYCR2 is an Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H. At physiologic concentrations, has higher specific activity in the presence of NADH. Involved in cellular response to oxidative stress. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+)[1][2][3][4].

  • Subcellular Localization

    Cytoplasm,Mitochondrion

  • Expression


    Tissue_Specificity: Detected in erythrocytes (at protein level) (PubMed:2722838, PubMed:6894153). Expressed in fetal brain (PubMed:25865492)

  • Isoforms & Post-Translational Modification

    Q96C36: 320 amino acids, molecular weight 33637 Da.

  • Subunit

    Homodecamer; composed of 5 homodimers (Probable)

  • SwissProt ID

    Q96C36

  • Synonyms

    HLD10; P5CR2

PYCR2 Antibody (YA7952) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20μg) , HepG2(lane 3(20μg) ,K562(lane 4(20μg)and Jurkat( lane 5(20μg) using PYCR2 Antibody (HY-P88268). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PYCR2 Antibody (YA7952) Product Image
    1/16
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • PYCR2 Antibody (YA7952) Product Image
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20μg) , HepG2(lane 3(20μg) ,K562(lane 4(20μg)and Jurkat( lane 5(20μg) using PYCR2 Antibody (HY-P88268). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • Experimental Validation Results for PYCR2 Antibody (YA7952)
  • PYCR2 Antibody (YA7952) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20μg) , HepG2(lane 3(20μg) ,K562(lane 4(20μg)and Jurkat( lane 5(20μg) using PYCR2 Antibody (HY-P88268). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • PYCR2 Antibody (YA7952) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PYCR2 Antibody (YA7952) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling PYCR2 with PYCR2 Antibody (HY-P88268) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PYCR2 Antibody (HY-P88268) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PYCR2 Antibody (YA7952) Product Image WB
  • PYCR2 Antibody (YA7952) Product Image ICC
  • PYCR2 Antibody (YA7952) Product Image ICC