RYK Antibody (YA2481)(PBS only)

Cat. No.: HY-P82736A: Data Sheet User Guide for Antibodies
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RYK Antibody (YA2481) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RYK.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RYK Antibody (YA2481) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RYK.

Host

Rabbit

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P34925

Gene ID

6259 [NCBI]

Immunogen

A synthetic peptide of human RYK aa575-589.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development

Subcellular Localization：Membrane; Single-pass type I membrane protein; Nucleus; Cytoplasm

Expression：
Tissue_specificity:Observed in all the tissues examined

Isoforms & Post-Translational Modification：P34925 has 2 isomers: P34925-1: 67815 Da (predicted); P34925-2: 68072 Da (predicted).
Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation

Subunit：Interacts with DVL1 (via PDZ domain)

Research Field

Synonyms

ERK 3; JTK5; JTK5A; Ryk; RYK1; Vik

Documentation

User Guide for Antibodies (1077 KB)