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DOK1 Antibody (YA2868)

(Synonyms: DOK1; Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62)
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Based on 1 Customer Validation

DOK1 Antibody (YA2868) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DOK1 Antibody (YA2868)
  • DOK1 Antibody (YA2868) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

DOK1 Antibody (YA2868) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOK1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human DOK1 aa430-481.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DOK1 Antibody (YA2868)
    Western blot analysis of extracts from Jurkat (lane2, 20μg), C2C12 (lane3, 20μg) and K562 (lane4, 20μg) using DOK1 Antibody (HY-P83123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    DOK1 is a DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer

  • Isoforms & Post-Translational Modification

    Q99704 has 3 isomers: Q99704-1: 52392 Da (predicted); Q99704-2: 19370 Da (predicted); Q99704-3: 37405 Da (predicted).
    Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS

  • Subunit

    Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3. Interacts with SRMS (via the SH2 and SH3 domains)

  • SwissProt ID

    Q99704

  • Gene ID
    1796 [NCBI]
  • Synonyms

    DOK1; Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62

  • Research Field

    Signal Transduction

DOK1 Antibody (YA2868) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), C2C12 (lane3, 20μg) and K562 (lane4, 20μg) using DOK1 Antibody (HY-P83123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • DOK1 Antibody (YA2868) Product Image
    1/16
  • Experimental Validation Results for DOK1 Antibody (YA2868)
  • DOK1 Antibody (YA2868) Product Image
  • Experimental Validation Results for DOK1 Antibody (YA2868)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), C2C12 (lane3, 20μg) and K562 (lane4, 20μg) using DOK1 Antibody (HY-P83123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DOK1 Antibody (YA2868)
  • DOK1 Antibody (YA2868) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane2, 20μg), C2C12 (lane3, 20μg) and K562 (lane4, 20μg) using DOK1 Antibody (HY-P83123). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • DOK1 Antibody (YA2868) Product Image WB