Proteasome 20S LMP7 Antibody (YA4953)
(Synonyms: PSMB8; LMP7; PSMB5i; RING10; Y2; Proteasome subunit beta type-8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting)Proteasome 20S LMP7 Antibody (YA4953) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Proteasome 20S LMP7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IHC-P; IHC-F; ICC/IF; IF-Tissue; FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:200-1000 | 1:200-1000 | 1:50-100 | 1:200-1000 | 1:50-100 |
Product Details
Proteasome 20S LMP7 Antibody (YA4953) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Proteasome 20S LMP7.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 23 kDa;
Recombinant human PSMB8 protein. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Proteasome 20S LMP7 is a The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes[1].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Induction:Up-regulated by IFNG/IFN-gamma and IRF1 (at protein level) . Up-regulated by TNF (at protein level) . Up-regulated by tetrodotoxin (TTX) in glial cells. Up-regulated in Crohn's bowel disease (CD) . Down-regulated by the selective inhibitor PR-957. Down-regulated in mature dendritic cells by HSV-1 infection. Up-regulated by heat shock treatment -
Isoforms & Post-Translational Modification
P28062 has 2 isomers: P28062-1: 30354 Da (predicted); P28062-2: 29770 Da (predicted).
Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity -
Subunit
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure.
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SwissProt ID
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Synonyms
PSMB8; LMP7; PSMB5i; RING10; Y2; Proteasome subunit beta type-8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting
Documentation
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