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  4. KIR3DL1 Antibody (YA4028)

KIR3DL1 Antibody (YA4028)

Cat. No.: HY-P84331
COA User Guide for Antibodies Technical Support

KIR3DL1 Antibody (YA4028) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KIR3DL1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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  • Documentation

Description

KIR3DL1 Antibody (YA4028) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KIR3DL1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight
Predicted band size: 49 kDa;
Observed band size: 49 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Rat, Monkey
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human KIR3DL1 (AA: extra 22-340) expressed in Hek293 cells.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
Purity affinity purified. Conjugation Non-conjugated
Modification Unmodified Isotype IgG2b
Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from Hela(lane 2(20ug) , HepG2(lane 3(20ug) and HL-60(lane 4(20ug) , HEK293(lane 5(20ug) using KIR3DL1 Antibody (HY-P84331) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Western blot analysis of extracts from SPC-A-1(lane 1(40μg) ), K562(lane 2(40μg) ), Raji(lane 3(40μg) )and A431(lane 4(40μg) ) using KIR3DL1 antibody(31373P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH,20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background
Function:Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis
Subcellular Localization:Cell membrane; Single-pass type I membrane protein
RRID
Synonyms
KIR; NKB1; NKAT3; NKB1B; NKAT-3; CD158E1; KIR3DL1/S1
Documentation

KIR3DL1 Antibody (YA4028) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
KIR3DL1 Antibody (YA4028)
Cat. No.:
HY-P84331
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