1. Antibodies
  2. Primary Antibodies
  3. Acetyl CoA synthetaseAntibody

Acetyl CoA synthetaseAntibody

Cat. No.: HY-P80483
COA User Guide for Antibodies

Acetyl CoA synthetaseAntibody is a non-conjugated and Rabbit origined monoclonal antibody about 79 kDa, targeting to Acetyl CoA synthetase. It can be used for WB,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Acetyl CoA synthetaseAntibody is a non-conjugated and Rabbit origined monoclonal antibody about 79 kDa, targeting to Acetyl CoA synthetase. It can be used for WB,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

Tag

Free

Molecular Weight

Predicted band size: 79 kDa

Purity

Protein A affinity purified.

Subcellular Location

Cytoplasm, Nucleus.

Conjugation

Non-conjugated

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

SwissProt ID

SwissProt: Q9NR19 Human;SwissProt: Q8BK97 Mouse;SwissProt: Q9QXG4 Mouse;SwissProt: D3ZFF9 Rat;SwissProt: D3ZUL6 Rat

Dilution Ratio

WB: 1:1,000 ;ICC/IF: 1:50-1:200

  • Western blot analysis of extracts from U87(lane 2(10μg) or (lane 3(20μg)) and HepG2(lane 4(10μg) or (lane 5(20μg)), using Acetyl CoA synthetase (HY-P80483) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80483, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB, ICC/IF

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Immunogen

Synthetic peptide corresponding to Human Acetyl CoA synthetase.AA range:101-145.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Acetyl CoA synthetaseAntibody
Cat. No.:
HY-P80483
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